tetano
Editor, Senior Moderator
Planta Med. 2018 Aug 21. doi: 10.1055/a-0690-9236. [Epub ahead of print]
[h=1]Lanostane Triterpenes from Gloeophyllum odoratum and Their Anti-Influenza Effects.[/h] Grienke U[SUP]1[/SUP], Zwirchmayr J[SUP]1[/SUP], Peintner U[SUP]2[/SUP], Urban E[SUP]3[/SUP], Zehl M[SUP]4[/SUP], Schmidtke M[SUP]5[/SUP], Rollinger JM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In an in vitro screening for anti-influenza agents from European polypores, the fruit body extract of Gloeophyllum odoratum dose-dependently inhibited the cytopathic effect of the H3N2 influenza virus A/Hong Kong/68 (HK/68) in Madin Darby canine kidney cells with a 50% inhibitory concentration (IC[SUB]50[/SUB]) of 15 ?g/mL, a noncytotoxic concentration. After a chromatographic work-up, eight lanostane triterpenes (1: -8: ) were isolated and their structures were elucidated based on high-resolution electrospray ionization mass spectrometry analyses, and one- and two-dimensional nuclear magnetic resonance experiments. Constituents 1: (gloeophyllin K) and 2: (gloeophyllin L) are reported here for the first time, and compounds 5: , 7: , and 8: have not been described for the investigated fungal material so far. The highest activity was determined for trametenolic acid B (3: ) against HK/68 and the 2009 pandemic H1N1 strain A/Jena/8178/09 with IC[SUB]50[/SUB] values of 14 and 11 ?M, respectively. In a plaque reduction assay, this compound was able to bind to cell-free viruses and to neutralize their infectivity.
PMID: 30130818 DOI: 10.1055/a-0690-9236
[h=1]Lanostane Triterpenes from Gloeophyllum odoratum and Their Anti-Influenza Effects.[/h] Grienke U[SUP]1[/SUP], Zwirchmayr J[SUP]1[/SUP], Peintner U[SUP]2[/SUP], Urban E[SUP]3[/SUP], Zehl M[SUP]4[/SUP], Schmidtke M[SUP]5[/SUP], Rollinger JM[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In an in vitro screening for anti-influenza agents from European polypores, the fruit body extract of Gloeophyllum odoratum dose-dependently inhibited the cytopathic effect of the H3N2 influenza virus A/Hong Kong/68 (HK/68) in Madin Darby canine kidney cells with a 50% inhibitory concentration (IC[SUB]50[/SUB]) of 15 ?g/mL, a noncytotoxic concentration. After a chromatographic work-up, eight lanostane triterpenes (1: -8: ) were isolated and their structures were elucidated based on high-resolution electrospray ionization mass spectrometry analyses, and one- and two-dimensional nuclear magnetic resonance experiments. Constituents 1: (gloeophyllin K) and 2: (gloeophyllin L) are reported here for the first time, and compounds 5: , 7: , and 8: have not been described for the investigated fungal material so far. The highest activity was determined for trametenolic acid B (3: ) against HK/68 and the 2009 pandemic H1N1 strain A/Jena/8178/09 with IC[SUB]50[/SUB] values of 14 and 11 ?M, respectively. In a plaque reduction assay, this compound was able to bind to cell-free viruses and to neutralize their infectivity.
PMID: 30130818 DOI: 10.1055/a-0690-9236