tetano
Editor, Senior Moderator
Lab Anim Res
. 2022 Sep 8;38(1):28.
doi: 10.1186/s42826-022-00138-z.
Elucidating the characteristics of Mx1 and resistance to influenza A virus subtype H1N1 in the newly developed KWM/Hym mice
Hajin Nam[SUP] 1 [/SUP], Boyoung Kim[SUP] 1 [/SUP], Avishekh Gautam[SUP] 2 [/SUP], Yoo Yeon Kim[SUP] 1 [/SUP], Eun Sun Park[SUP] 1 [/SUP], Jong Sun Lee[SUP] 1 [/SUP], Hyung-Joo Kwon[SUP] 2 3 [/SUP], Je Kyung Seong[SUP] 4 [/SUP], Jun Gyo Suh[SUP] 5 6 [/SUP]
Affiliations
Abstract
Background: Inbred mice have several advantages, including genetic similarity to humans, a well-established gene manipulation system, and strong tolerance to inbreeding. However, inbred mice derived from a limited genetic pool have a small genetic diversity. Thus, the development of new inbred strains from wild mice is needed to overcome this limitation. Hence, in this study, we used a new strain of inbred mice called KWM/Hym. We sequenced the Mx1 gene to elucidate the genetic diversities of KWM/Hym mice and observed the biological alterations of the Mx1 protein upon influenza A infection.
Results: The Mx1 gene in KWM/Hym mice had 2, 4, and 38 nucleotide substitutions compared to those in the Mx1 gene in A2G, CAST/EiJ, and Mus spretus mice, respectively. Moreover, the Mx1 protein in KWM/Hym mice had 2 and 25 amino acid substitutions compared to those in the Mx1 protein in CAST/EiJ and M. spretus mice, respectively. To elucidate the function of the Mx1 protein, we inoculated the influenza A virus (A/WSN/1933) in KWM/Hym mice. Nine days after infection, all infected KWM/Hym mice survived without any weight loss. Four days after infection, the lungs of the infected KWM/Hym mice showed mild alveolitis and loss of bronchiolar epithelium; however, the pulmonary viral titers of the infected KWM/Hym mice were significantly lower than that in the infected BALB/c mice (2.17 × plaque-forming units mL[SUP]-1[/SUP]).
Conclusions: Our results demonstrate that the KWM/Hym mice are resistant to influenza A virus infection. Further, these mice can be used as a model organism to understand the mechanism of influenza A virus susceptibility.
Keywords: Alveolitis; Influenza A (H1N1) virus; KWM/Hym mice; Mx1 gene.
. 2022 Sep 8;38(1):28.
doi: 10.1186/s42826-022-00138-z.
Elucidating the characteristics of Mx1 and resistance to influenza A virus subtype H1N1 in the newly developed KWM/Hym mice
Hajin Nam[SUP] 1 [/SUP], Boyoung Kim[SUP] 1 [/SUP], Avishekh Gautam[SUP] 2 [/SUP], Yoo Yeon Kim[SUP] 1 [/SUP], Eun Sun Park[SUP] 1 [/SUP], Jong Sun Lee[SUP] 1 [/SUP], Hyung-Joo Kwon[SUP] 2 3 [/SUP], Je Kyung Seong[SUP] 4 [/SUP], Jun Gyo Suh[SUP] 5 6 [/SUP]
Affiliations
- PMID: 36076303
- DOI: 10.1186/s42826-022-00138-z
Abstract
Background: Inbred mice have several advantages, including genetic similarity to humans, a well-established gene manipulation system, and strong tolerance to inbreeding. However, inbred mice derived from a limited genetic pool have a small genetic diversity. Thus, the development of new inbred strains from wild mice is needed to overcome this limitation. Hence, in this study, we used a new strain of inbred mice called KWM/Hym. We sequenced the Mx1 gene to elucidate the genetic diversities of KWM/Hym mice and observed the biological alterations of the Mx1 protein upon influenza A infection.
Results: The Mx1 gene in KWM/Hym mice had 2, 4, and 38 nucleotide substitutions compared to those in the Mx1 gene in A2G, CAST/EiJ, and Mus spretus mice, respectively. Moreover, the Mx1 protein in KWM/Hym mice had 2 and 25 amino acid substitutions compared to those in the Mx1 protein in CAST/EiJ and M. spretus mice, respectively. To elucidate the function of the Mx1 protein, we inoculated the influenza A virus (A/WSN/1933) in KWM/Hym mice. Nine days after infection, all infected KWM/Hym mice survived without any weight loss. Four days after infection, the lungs of the infected KWM/Hym mice showed mild alveolitis and loss of bronchiolar epithelium; however, the pulmonary viral titers of the infected KWM/Hym mice were significantly lower than that in the infected BALB/c mice (2.17 × plaque-forming units mL[SUP]-1[/SUP]).
Conclusions: Our results demonstrate that the KWM/Hym mice are resistant to influenza A virus infection. Further, these mice can be used as a model organism to understand the mechanism of influenza A virus susceptibility.
Keywords: Alveolitis; Influenza A (H1N1) virus; KWM/Hym mice; Mx1 gene.