tetano
Editor, Senior Moderator
Journal of Proteomics
Available online 12 July 2013
In Press, Accepted Manuscript ? Note to users
Cover image
Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus ☆
Open Access Article
Xiaopeng Wua, b,
Hailong Wanga, b,
Bai Lua, b,
Yu Yanga, b,
Zeyu Sund,
Yan Yana, b,
Jiyong Zhoua, b, c, Corresponding author contact information, E-mail the corresponding author
a Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, PR China
b State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, PR China
c Collaberative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, PR China
d Zhejiang California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, PR China
http://dx.doi.org/10.1016/j.jprot.2013.06.037, How to Cite or Link Using DOI
Permissions & Reprints
Highlights
?
Differential mitochondrial proteomes of H3N2 SIV-infected A549 cells were analyzed.
?
We used two-dimensional gel electrophoresis and MALDI-TOF/TOF analysis.
?
Twenty-four proteins showed altered expression following H3N2 SIV infection.
?
APOL2 was upregulated in cytosol and translocated to mitochondria during infection.
?
Western blot analysis and confocal microscopy were used to verify the findings.
Abstract
Swine influenza viruses (SIV) are zoonotic pathogens that pose a potential threat to human health. In this study, we analyzed the differential mitochondrial proteomes of H3N2 SIV-infected human lung A549 cells using two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis. In the comparative analysis, 24 altered proteins (13 upregulated and 11 downregulated) were identified in the mitochondria of H3N2 SIV-infected cells; these proteins were involved in cell-to-cell signaling and interaction, cellular movement, and post-translational modification. Moreover, the transcriptional profiles of 16 genes corresponding to the identified proteins were estimated by real time RT-PCR. IPA analysis suggested that the differentially expressed proteins were clustered primarily into the mammalian target of rapamycin (mTOR) and D-glucose signaling pathways. In addition, oxidative phosphorylation and integrin signaling appeared to be major pathways modulated in the mitochondria of infected cells. We further demonstrated that apolipoprotein L2 was upregulated in the cytoplasm and translocated to mitochondria during virus infection. These results were verified by Western blot analysis coupled with confocal microscopy. Collectively, the mitochondrial proteome data provide insights to further understand the underlying mechanisms of H3N2 SIV cross-species infection.
Biological Significance
In recent years, proteomics has emerged as an indispensable tool to unveil the complex molecular events in virology. we firstly perform mitochondrial proteomic profiles of human cells infected with H3N2 subtype SIV to understand virus-host interactions, and 24 differentially expressed proteins in mitochondrial proteomes were identified in SIV-infected cells. The proteins that were identified to have differential expression were involved in cell-to-cell signaling and interaction, post-translational modification, cell morphology, cellular assembly, cell death, and energy production. Furthermore, Western blot analysis and a confocal assay further demonstrated that the cellular protein APLO2 partially co-localized with mitochondria after virus infection. This is very important discover in the underlying replication and pathogenesis of SIV and also provide a potential target clue for the design of anti-SIV drugs. Our results will inspire basic study on SIV infection and drive the understanding for replication and pathogenesis of SIV to control this disease.
http://www.sciencedirect.com/science/article/pii/S1874391913003862
Available online 12 July 2013
In Press, Accepted Manuscript ? Note to users
Cover image
Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus ☆
Open Access Article
Xiaopeng Wua, b,
Hailong Wanga, b,
Bai Lua, b,
Yu Yanga, b,
Zeyu Sund,
Yan Yana, b,
Jiyong Zhoua, b, c, Corresponding author contact information, E-mail the corresponding author
a Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, PR China
b State Key Laboratory, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, PR China
c Collaberative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, PR China
d Zhejiang California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, PR China
http://dx.doi.org/10.1016/j.jprot.2013.06.037, How to Cite or Link Using DOI
Permissions & Reprints
Highlights
?
Differential mitochondrial proteomes of H3N2 SIV-infected A549 cells were analyzed.
?
We used two-dimensional gel electrophoresis and MALDI-TOF/TOF analysis.
?
Twenty-four proteins showed altered expression following H3N2 SIV infection.
?
APOL2 was upregulated in cytosol and translocated to mitochondria during infection.
?
Western blot analysis and confocal microscopy were used to verify the findings.
Abstract
Swine influenza viruses (SIV) are zoonotic pathogens that pose a potential threat to human health. In this study, we analyzed the differential mitochondrial proteomes of H3N2 SIV-infected human lung A549 cells using two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis. In the comparative analysis, 24 altered proteins (13 upregulated and 11 downregulated) were identified in the mitochondria of H3N2 SIV-infected cells; these proteins were involved in cell-to-cell signaling and interaction, cellular movement, and post-translational modification. Moreover, the transcriptional profiles of 16 genes corresponding to the identified proteins were estimated by real time RT-PCR. IPA analysis suggested that the differentially expressed proteins were clustered primarily into the mammalian target of rapamycin (mTOR) and D-glucose signaling pathways. In addition, oxidative phosphorylation and integrin signaling appeared to be major pathways modulated in the mitochondria of infected cells. We further demonstrated that apolipoprotein L2 was upregulated in the cytoplasm and translocated to mitochondria during virus infection. These results were verified by Western blot analysis coupled with confocal microscopy. Collectively, the mitochondrial proteome data provide insights to further understand the underlying mechanisms of H3N2 SIV cross-species infection.
Biological Significance
In recent years, proteomics has emerged as an indispensable tool to unveil the complex molecular events in virology. we firstly perform mitochondrial proteomic profiles of human cells infected with H3N2 subtype SIV to understand virus-host interactions, and 24 differentially expressed proteins in mitochondrial proteomes were identified in SIV-infected cells. The proteins that were identified to have differential expression were involved in cell-to-cell signaling and interaction, post-translational modification, cell morphology, cellular assembly, cell death, and energy production. Furthermore, Western blot analysis and a confocal assay further demonstrated that the cellular protein APLO2 partially co-localized with mitochondria after virus infection. This is very important discover in the underlying replication and pathogenesis of SIV and also provide a potential target clue for the design of anti-SIV drugs. Our results will inspire basic study on SIV infection and drive the understanding for replication and pathogenesis of SIV to control this disease.
http://www.sciencedirect.com/science/article/pii/S1874391913003862