Giuseppe
Emeritus
The Rab11 pathway is required for influenza A viral budding and filament formation. (J Virol., abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
J Virol. 2010 Mar 31. [Epub ahead of print]
The Rab11 pathway is required for influenza A viral budding and filament formation.
Bruce EA, Digard P, Stuart AD. - Division of Virology, Department of Pathology, University of Cambridge, Tennis Court, Road, Cambridge CB2 1QP, United Kingdom.
Influenza A virus buds through the apical plasma membrane, forming enveloped virus particles that can take the shape of pleiomorphic spheres or vastly elongated filaments. For either type of virion, the factors responsible for separation of viral and cell membranes are not known. We find that cellular Rab11 (a small GTP-binding protein involved in endocytic recycling) and Rab11-Family-Interacting-Protein-3 (FIP3, which plays a role in membrane trafficking and regulation of actin dynamics) are both required to support the formation of filamentous virions, while Rab11 is additionally involved in the final budding step of spherical particles. Cells transfected with Rab 11 GTP-cycling mutants or depleted of Rab11 or FIP3 content by siRNA treatment lost the ability to form viral filaments. Depletion of Rab11 resulted in up to a 100-fold decrease in titre of spherical virus released from cells. Scanning electron microscopy of Rab11 depleted cells showed high densities of virus particles apparently stalled in the process of budding. Transmission electron microscopy of thin sections confirmed that Rab11 depletion resulted in significant numbers of abnormally formed virus particles that had failed to pinch off from the plasma membrane. Based on these findings, we see a clear role for a Rab11-mediated pathway in influenza virus morphogenesis and budding.
PMID: 20357086 [PubMed - as supplied by publisher]
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[Source: US National Library of Medicine, (LINK). Edited.]
J Virol. 2010 Mar 31. [Epub ahead of print]
The Rab11 pathway is required for influenza A viral budding and filament formation.
Bruce EA, Digard P, Stuart AD. - Division of Virology, Department of Pathology, University of Cambridge, Tennis Court, Road, Cambridge CB2 1QP, United Kingdom.
Influenza A virus buds through the apical plasma membrane, forming enveloped virus particles that can take the shape of pleiomorphic spheres or vastly elongated filaments. For either type of virion, the factors responsible for separation of viral and cell membranes are not known. We find that cellular Rab11 (a small GTP-binding protein involved in endocytic recycling) and Rab11-Family-Interacting-Protein-3 (FIP3, which plays a role in membrane trafficking and regulation of actin dynamics) are both required to support the formation of filamentous virions, while Rab11 is additionally involved in the final budding step of spherical particles. Cells transfected with Rab 11 GTP-cycling mutants or depleted of Rab11 or FIP3 content by siRNA treatment lost the ability to form viral filaments. Depletion of Rab11 resulted in up to a 100-fold decrease in titre of spherical virus released from cells. Scanning electron microscopy of Rab11 depleted cells showed high densities of virus particles apparently stalled in the process of budding. Transmission electron microscopy of thin sections confirmed that Rab11 depletion resulted in significant numbers of abnormally formed virus particles that had failed to pinch off from the plasma membrane. Based on these findings, we see a clear role for a Rab11-mediated pathway in influenza virus morphogenesis and budding.
PMID: 20357086 [PubMed - as supplied by publisher]
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