Giuseppe
Emeritus
J Virol. 2009 Jan 21. [Epub ahead of print]
Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication.
Ozawa M, Maeda J, Iwatsuki-Horimoto K, Watanabe S, Goto H, Horimoto T, Kawaoka Y. - Division of Virology, Department of Microbiology and Immunology, and International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639; ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012; Laboratory of Prion Diseases, Department of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-818, Japan; and Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706.
The mechanism by which the influenza A virus genome is packaged into virions is not fully understood.
The coding and non-coding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified.
Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions.
We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.
PMID: 19158245 [PubMed - as supplied by publisher]
-
------
-
Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication.
Ozawa M, Maeda J, Iwatsuki-Horimoto K, Watanabe S, Goto H, Horimoto T, Kawaoka Y. - Division of Virology, Department of Microbiology and Immunology, and International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639; ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012; Laboratory of Prion Diseases, Department of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-818, Japan; and Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706.
The mechanism by which the influenza A virus genome is packaged into virions is not fully understood.
The coding and non-coding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified.
Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions.
We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.
PMID: 19158245 [PubMed - as supplied by publisher]
-
------
-