Giuseppe
Emeritus
J Virol Methods. 2008 Aug 18. [Epub ahead of print]
Rapid molecular detection of the H275Y oseltamivir resistance gene mutation in circulating influenza A (H1N1) viruses.
Carr MJ, Sayre N, Duffy M, Connell J, Hall WW. - National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
The neuraminidase inhibitor (NAI), oseltamivir has been stockpiled around the world as an antiviral therapeutic option for an anticipated influenza pandemic.
In early 2008, drug susceptibility surveillance of influenza viruses in Europe revealed that some influenza A viruses (subtype H1N1) circulating during the winter season of 2007 and 2008 were resistant to the NAI, oseltamivir.
The genetic marker of viral drug resistance arises through a cytosine to thymine mutation in the influenza A/H1N1 neuraminidase 1 gene resulting in a histidine to tyrosine substitution at amino acid position 275 in the sialidase active site (H275Y in N1 nomenclature).
Current methods to detect this mutation involve an end-point reverse transcription polymerase chain reaction followed by nucleotide sequencing.
While accurate, this approach has the limitation of being time-consuming, labour-intensive and expensive.
PMID: 18718489 [PubMed - as supplied by publisher
-
http://www.ncbi.nlm.nih.gov/pubmed/18718489
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Rapid molecular detection of the H275Y oseltamivir resistance gene mutation in circulating influenza A (H1N1) viruses.
Carr MJ, Sayre N, Duffy M, Connell J, Hall WW. - National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
The neuraminidase inhibitor (NAI), oseltamivir has been stockpiled around the world as an antiviral therapeutic option for an anticipated influenza pandemic.
In early 2008, drug susceptibility surveillance of influenza viruses in Europe revealed that some influenza A viruses (subtype H1N1) circulating during the winter season of 2007 and 2008 were resistant to the NAI, oseltamivir.
The genetic marker of viral drug resistance arises through a cytosine to thymine mutation in the influenza A/H1N1 neuraminidase 1 gene resulting in a histidine to tyrosine substitution at amino acid position 275 in the sialidase active site (H275Y in N1 nomenclature).
Current methods to detect this mutation involve an end-point reverse transcription polymerase chain reaction followed by nucleotide sequencing.
While accurate, this approach has the limitation of being time-consuming, labour-intensive and expensive.
PMID: 18718489 [PubMed - as supplied by publisher
-
http://www.ncbi.nlm.nih.gov/pubmed/18718489
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