J Virol Methods. Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets.

[Giuseppe]

Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets. (J Virol Methods., abstract, edited)

10. J Virol Methods. 2010 Jan 25. [Epub ahead of print]

Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets.

Chander Y, Jindal N, Stallknecht DE, Goyal SM. - Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota 55108.

An RT-PCR based method was developed using subtype specific overlapping primers to obtain full length amplification of neuraminidase (NA) gene from all subtypes (N1-N9) of influenza A viruses. This method was validated using reference strains of avian influenza viruses (AIV) (N1-N9), human influenza viruses (N1and N2), and swine influenza viruses (N1-N3). Amplification of the NA gene was obtained with all viruses tested. Additionally, 200 field isolates of AIV from wild birds were tested by this method and the NA gene was amplified in all isolates. The NA subtype of all 200 isolates was determined by further sequencing of the amplified NA genes and all sequences were submitted to GenBank. The method described in this paper can be used to determine subtype of influenza isolates as well as their evolution and mutations if any, in the NA gene.

Copyright ? 2010. Published by Elsevier B.V.

PMID: 20109495 [PubMed - as supplied by publisher]
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[gsgs]

Re: J Virol Methods. Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets.

HA and NA subtypes are becoming less important
while other segments are easy to sequence too
and not much less important/interesting (IMO)