Giuseppe
Emeritus
J Virol. 2009 Feb 11. [Epub ahead of print]
Kinetics of immune responses to influenza virus-like particles and dose-dependence of protection with a single vaccination.
Quan FS, Yoo DG, Song JM, Clements JD, Compans RW, Kang SM. - Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322; and Department of Microbiology and Immunology, Tulane University Medical Center, New Orleans, Louisiana 701122.
The format of influenza virus-like particles (VLPs) as a non-replicating particulate vaccine candidate is a promising alternative to conventional egg-based vaccines.
In this study, we have investigated the detailed kinetics of immune responses and protective efficacy after a single intranasal immunization with different doses of VLPs alone or in the presence of E. coli mutant heat labile enterotoxin (LT(R192G)) or cholera toxin subunit B (CTB) as adjuvants.
Analysis of immune responses showed differential kinetics in a VLP antigen-dose dependent manner and dynamic changes in the ratios of antibody IgG isotypes over the time course.
Protection against lethal challenge was observed with a single immunization with influenza VLPs even without adjuvant.
Addition of adjuvant showed significant antigen sparing effects with improved protective efficacy. The protective immune responses, efficacies of protection, and antigen sparing effects were significantly improved by a second immunization as determined by the levels of neutralizing antibodies, morbidity post challenge, lung viral titers and inflammatory cytokines.
Our results are informative for better understanding the protective immunity induced by a single dose or two doses of influenza VLPs, which is dependent on antigen dosage and the presence of adjuvant, and will provide insights into designing effective vaccines based on VLPs.
PMID: 19211762 [PubMed - as supplied by publisher]
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Kinetics of immune responses to influenza virus-like particles and dose-dependence of protection with a single vaccination.
Quan FS, Yoo DG, Song JM, Clements JD, Compans RW, Kang SM. - Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322; and Department of Microbiology and Immunology, Tulane University Medical Center, New Orleans, Louisiana 701122.
The format of influenza virus-like particles (VLPs) as a non-replicating particulate vaccine candidate is a promising alternative to conventional egg-based vaccines.
In this study, we have investigated the detailed kinetics of immune responses and protective efficacy after a single intranasal immunization with different doses of VLPs alone or in the presence of E. coli mutant heat labile enterotoxin (LT(R192G)) or cholera toxin subunit B (CTB) as adjuvants.
Analysis of immune responses showed differential kinetics in a VLP antigen-dose dependent manner and dynamic changes in the ratios of antibody IgG isotypes over the time course.
Protection against lethal challenge was observed with a single immunization with influenza VLPs even without adjuvant.
Addition of adjuvant showed significant antigen sparing effects with improved protective efficacy. The protective immune responses, efficacies of protection, and antigen sparing effects were significantly improved by a second immunization as determined by the levels of neutralizing antibodies, morbidity post challenge, lung viral titers and inflammatory cytokines.
Our results are informative for better understanding the protective immunity induced by a single dose or two doses of influenza VLPs, which is dependent on antigen dosage and the presence of adjuvant, and will provide insights into designing effective vaccines based on VLPs.
PMID: 19211762 [PubMed - as supplied by publisher]
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