Giuseppe
Emeritus
J Virol. 2009 Mar 25. [Epub ahead of print]
Influenza A virus lacking M2 protein as a live attenuated vaccine.
Watanabe S, Watanabe T, Kawaoka Y. - Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; International Research Center for Infectious Diseases, Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639.
Mutant influenza virus that lacks the transmembrane and cytoplasmic tail domains of M2 (M2 knock out; M2KO) is attenuated in both cell culture and mice. Here, we examined the potency of M2KO influenza virus as a live attenuated influenza vaccine.
M2KO virus grew as efficiently as the wild-type virus in cells stably expressing the wild-type M2, indicating the feasibility of efficient vaccine production.
Mice intranasally vaccinated with M2KO virus developed protective immune responses and survived lethal challenge with wild-type virus, suggesting that the M2KO virus has potential as a live attenuated vaccine.
PMID: 19321619 [PubMed - as supplied by publisher]
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Influenza A virus lacking M2 protein as a live attenuated vaccine.
Watanabe S, Watanabe T, Kawaoka Y. - Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; International Research Center for Infectious Diseases, Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639.
Mutant influenza virus that lacks the transmembrane and cytoplasmic tail domains of M2 (M2 knock out; M2KO) is attenuated in both cell culture and mice. Here, we examined the potency of M2KO influenza virus as a live attenuated influenza vaccine.
M2KO virus grew as efficiently as the wild-type virus in cells stably expressing the wild-type M2, indicating the feasibility of efficient vaccine production.
Mice intranasally vaccinated with M2KO virus developed protective immune responses and survived lethal challenge with wild-type virus, suggesting that the M2KO virus has potential as a live attenuated vaccine.
PMID: 19321619 [PubMed - as supplied by publisher]
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