Giuseppe
Emeritus
Cytotoxic T cells are the predominant players providing cross-protective immunity induced by {gamma}-irradiated influenza A viruses. (J Virol., abstract, edited)
7. J Virol. 2010 Feb 17. [Epub ahead of print]
Cytotoxic T cells are the predominant players providing cross-protective immunity induced by {gamma}-irradiated influenza A viruses.
Furuya Y, Chan J, Regner M, Lobigs M, Koskinen A, Kok T, Manavis J, Li P, M?llbacher A, Alsharifi M. - Viral Immunology and Molecular Virology, The John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia; Microbiology and Infectious Diseases, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia.
We have previously demonstrated that a single dose of non-adjuvanted intranasal gamma-irradiated influenza A virus can provide robust protection in mice against both homologous and heterosubtypic challenges, including an H5N1 avian strain. We investigated the mechanism behind the observed cross-protection to define which arms of the adaptive immune response are involved in mediating this protection. Studies with gene knock-out mice show the cross-protective immunity to be mainly mediated by T cells and dependent on the cytolytic effector molecule perforin. Adoptive transfer of memory T cells from immunized mice, but not memory B cells, protected na?ve recipients against lethal heterosubtypic influenza challenge. Furthermore, gamma-irradiated influenza viruses induced cross-reactive Tc cell responses, but not cross-neutralizing or cross-protective antibodies. In addition, histological analysis showed reduced lung inflammation in vaccinated mice compared to unvaccinated controls following heterosubtypic challenge. This reduced inflammation is associated with enhanced early recruitment of T cells, both CD4(+) and CD8(+), and early influenza-specific cytotoxic T cell responses. Therefore, cross-protective immunity induced by vaccination with gamma-irradiated influenza A virus is mainly mediated by Tc cell responses.
PMID: 20164231 [PubMed - as supplied by publisher]
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7. J Virol. 2010 Feb 17. [Epub ahead of print]
Cytotoxic T cells are the predominant players providing cross-protective immunity induced by {gamma}-irradiated influenza A viruses.
Furuya Y, Chan J, Regner M, Lobigs M, Koskinen A, Kok T, Manavis J, Li P, M?llbacher A, Alsharifi M. - Viral Immunology and Molecular Virology, The John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia; Microbiology and Infectious Diseases, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia.
We have previously demonstrated that a single dose of non-adjuvanted intranasal gamma-irradiated influenza A virus can provide robust protection in mice against both homologous and heterosubtypic challenges, including an H5N1 avian strain. We investigated the mechanism behind the observed cross-protection to define which arms of the adaptive immune response are involved in mediating this protection. Studies with gene knock-out mice show the cross-protective immunity to be mainly mediated by T cells and dependent on the cytolytic effector molecule perforin. Adoptive transfer of memory T cells from immunized mice, but not memory B cells, protected na?ve recipients against lethal heterosubtypic influenza challenge. Furthermore, gamma-irradiated influenza viruses induced cross-reactive Tc cell responses, but not cross-neutralizing or cross-protective antibodies. In addition, histological analysis showed reduced lung inflammation in vaccinated mice compared to unvaccinated controls following heterosubtypic challenge. This reduced inflammation is associated with enhanced early recruitment of T cells, both CD4(+) and CD8(+), and early influenza-specific cytotoxic T cell responses. Therefore, cross-protective immunity induced by vaccination with gamma-irradiated influenza A virus is mainly mediated by Tc cell responses.
PMID: 20164231 [PubMed - as supplied by publisher]
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