tetano
Editor, Senior Moderator
J Mol Diagn. 2020 Apr 7. pii: S1525-1578(20)30090-8. doi: 10.1016/j.jmoldx.2020.03.006. [Epub ahead of print]
Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2.
Park GS[SUP]1[/SUP], Ku K[SUP]2[/SUP], Baek SH[SUP]3[/SUP], Kim SJ[SUP]2[/SUP], Kim SI[SUP]4[/SUP], Kim BT[SUP]2[/SUP], Maeng JS[SUP]5[/SUP].
Author information
Abstract
Epidemics of Coronavirus Disease 2019 (COVID-19) now have more than 100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by RT-qPCR methods, but the capacity of RT-qPCR methods is limited by its requirement of high-level facilities and instruments. Here, we developed and evaluated RT-LAMP assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human Coronaviruses was not observed. We also adapted a colorimetric detection method for our RT-LAMP assay so that the tests potentially performed in higher throughput.
Copyright ? 2020. Published by Elsevier Inc.
PMID:32276051PMCID:PMC7144851DOI:10.1016/j.jmoldx.2020.03.006
Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2.
Park GS[SUP]1[/SUP], Ku K[SUP]2[/SUP], Baek SH[SUP]3[/SUP], Kim SJ[SUP]2[/SUP], Kim SI[SUP]4[/SUP], Kim BT[SUP]2[/SUP], Maeng JS[SUP]5[/SUP].
Author information
Abstract
Epidemics of Coronavirus Disease 2019 (COVID-19) now have more than 100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by RT-qPCR methods, but the capacity of RT-qPCR methods is limited by its requirement of high-level facilities and instruments. Here, we developed and evaluated RT-LAMP assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human Coronaviruses was not observed. We also adapted a colorimetric detection method for our RT-LAMP assay so that the tests potentially performed in higher throughput.
Copyright ? 2020. Published by Elsevier Inc.
PMID:32276051PMCID:PMC7144851DOI:10.1016/j.jmoldx.2020.03.006