Giuseppe
Emeritus
[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
J Microbiol Immunol Infect. 2013 Dec 30. pii: S1684-1182(13)00201-6. doi: 10.1016/j.jmii.2013.09.010. [Epub ahead of print]
Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus.
Mok CK<SUP>1</SUP>, Chang SC<SUP>2</SUP>, Chen GW<SUP>3</SUP>, Lo YL<SUP>2</SUP>, Chen SJ<SUP>4</SUP>, Wu HS<SUP>5</SUP>, Liu MT<SUP>5</SUP>, Chang FY<SUP>5</SUP>, Lin TY<SUP>6</SUP>, Shih SR<SUP>7</SUP>.
Author information: <SUP>1</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>2</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC. <SUP>3</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>4</SUP>Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC; Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>5</SUP>Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan, ROC. <SUP>6</SUP>Ministry of Health and Welfare, Executive Yuan, Taipei, Taiwan, ROC. <SUP>7</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC. Electronic address: srshih@mail.cgu.edu.tw.
Abstract
Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS: Avian influenza A virus, H7N9, Oseltamivir-resistant marker, Pyrosequencing
PMID: 24388586 [PubMed - as supplied by publisher]
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J Microbiol Immunol Infect. 2013 Dec 30. pii: S1684-1182(13)00201-6. doi: 10.1016/j.jmii.2013.09.010. [Epub ahead of print]
Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus.
Mok CK<SUP>1</SUP>, Chang SC<SUP>2</SUP>, Chen GW<SUP>3</SUP>, Lo YL<SUP>2</SUP>, Chen SJ<SUP>4</SUP>, Wu HS<SUP>5</SUP>, Liu MT<SUP>5</SUP>, Chang FY<SUP>5</SUP>, Lin TY<SUP>6</SUP>, Shih SR<SUP>7</SUP>.
Author information: <SUP>1</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>2</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC. <SUP>3</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>4</SUP>Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC; Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan, ROC. <SUP>5</SUP>Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan, ROC. <SUP>6</SUP>Ministry of Health and Welfare, Executive Yuan, Taipei, Taiwan, ROC. <SUP>7</SUP>Research Center for Emerging Viral Infections, Chang Gung University and Clinical Virology Laboratory, Chang Gung Memorial Hospital, Taiwan Centers for Disease Control Collaborating Laboratory of Virology, Taoyuan, Taiwan, ROC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC. Electronic address: srshih@mail.cgu.edu.tw.
Abstract
Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS: Avian influenza A virus, H7N9, Oseltamivir-resistant marker, Pyrosequencing
PMID: 24388586 [PubMed - as supplied by publisher]
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