tetano
Editor, Senior Moderator
J Med Virol
. 2021 Oct 21.
doi: 10.1002/jmv.27413. Online ahead of print.
THE EVALUATION POTENTIAL GLOBAL IMPACT OF THE N501Y MUTATION IN SARS-COV-2 POSITIVE PATIENTS
Selen Zeliha Mart Komurcu[SUP] 1 [/SUP], Yakup Artik[SUP] 2 [/SUP], Nevra Pelin Cesur[SUP] 2 [/SUP], Arzu Tanriverdi[SUP] 1 [/SUP], Derya Cakir Erdogan[SUP] 1 [/SUP], Sule Celik[SUP] 1 [/SUP], Elif Yilmaz Gulec[SUP] 1 [/SUP]
Affiliations
Abstract
Rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations are significant to control the contagion and spread rate of the virus. We aimed to evaluate the N501Y mutation rate in randomly chosen positive patients with polymerase chain reaction (PCR). The evaluation and analysis of the data in terms of public health will contribute to the literature on the global pandemic that affects our society with a retrospective approach in cases with mutations. Public health will take the necessary precautions and evaluate the current situation. The N501Y mutation detected in patients with positive Covid-19 PCR test results. The positive samples were examined based on the 6-carboxy-fluorescein (FAM) channel in reverse transcription PCR (RT-PCR) quantitation cycle (Cq) values as low Cq (<25), medium Cq (25-32), and high Cq (32-38) groups. In the study, 2757 (19.7%) of 13,972 cases were detected as mutation suspects and 159 (5.8%) of them were found to have mutations. The ages of the cases with mutations ranged from 1 to 88 years (mean age of 40.99±17.55). The 49.7% (n=79) of the cases with mutations were male, and 50.3% (n=80) were female. When the RT-PCR-Cq results were examined, it was seen that it varied between 11.3 and 35.03 with an average 20.75±3.32. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; N501Y mutation; Polymerase chain reaction (PCR); Reverse transcription PCR (RT-PCR); SARS-CoV-2.
. 2021 Oct 21.
doi: 10.1002/jmv.27413. Online ahead of print.
THE EVALUATION POTENTIAL GLOBAL IMPACT OF THE N501Y MUTATION IN SARS-COV-2 POSITIVE PATIENTS
Selen Zeliha Mart Komurcu[SUP] 1 [/SUP], Yakup Artik[SUP] 2 [/SUP], Nevra Pelin Cesur[SUP] 2 [/SUP], Arzu Tanriverdi[SUP] 1 [/SUP], Derya Cakir Erdogan[SUP] 1 [/SUP], Sule Celik[SUP] 1 [/SUP], Elif Yilmaz Gulec[SUP] 1 [/SUP]
Affiliations
- PMID: 34676574
- DOI: 10.1002/jmv.27413
Abstract
Rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations are significant to control the contagion and spread rate of the virus. We aimed to evaluate the N501Y mutation rate in randomly chosen positive patients with polymerase chain reaction (PCR). The evaluation and analysis of the data in terms of public health will contribute to the literature on the global pandemic that affects our society with a retrospective approach in cases with mutations. Public health will take the necessary precautions and evaluate the current situation. The N501Y mutation detected in patients with positive Covid-19 PCR test results. The positive samples were examined based on the 6-carboxy-fluorescein (FAM) channel in reverse transcription PCR (RT-PCR) quantitation cycle (Cq) values as low Cq (<25), medium Cq (25-32), and high Cq (32-38) groups. In the study, 2757 (19.7%) of 13,972 cases were detected as mutation suspects and 159 (5.8%) of them were found to have mutations. The ages of the cases with mutations ranged from 1 to 88 years (mean age of 40.99±17.55). The 49.7% (n=79) of the cases with mutations were male, and 50.3% (n=80) were female. When the RT-PCR-Cq results were examined, it was seen that it varied between 11.3 and 35.03 with an average 20.75±3.32. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; N501Y mutation; Polymerase chain reaction (PCR); Reverse transcription PCR (RT-PCR); SARS-CoV-2.