Giuseppe
Emeritus
[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
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Excretion of West Nile Virus in Urine During Acute Infection
Luisa Barzon 1,2, Monia Pacenti 2, Elisa Franchin 1,2, Silvana Pagni 1,2, Thomas Martello 2, Margherita Cattai 2, Riccardo Cusinato 2 and Giorgio Pal? 1,2
Author Affiliations: <SUP>1</SUP>Department of Molecular Medicine, University of Padova, I-35121 Padova, Italy <SUP>2</SUP>Regional Reference Laboratory for Infectious Diseases, Microbiology and Virology Unit, Padova University Hospital, I-35128 Padova, Italy
Corresponding authors contact information: Luisa Barzon, MD, Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, I-35121 Padova, Italy. Tel. +39-0498218946; Fax +39-0498272355; Email luisa.barzon@unipd.it; Giorgio Pal?, MD, Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, I-35121 Padova, Italy. Tel. +39-0498272350; Fax +39-0498272355; Email giorgio.palu@unipd.it
Abstract
Detection of West Nile virus (WNV) RNA in urine has been anecdotally described and proposed for the diagnosis of WNV infection. This study reports the application of real-time RT-PCR for the detection of WNV RNA in urine as a routine procedure in support to diagnosis of WNV infection during the large outbreak that occurred in North-Eastern Italy in 2012. Fourteen out of 32 (43.8%) patients with symptomatic WNV infection, i.e., neuroinvasive disease and fever, had detectable WNV RNA in urine at the time of diagnosis, at a higher rate and load and for longer time than detection of WNV RNA in blood. At variance, detection of WNV RNA in urine was less frequent (2 out of 14, 14.2%) in blood donors in whom WNV infection was identified by WNV NAAT screening. Infectious virus was isolated from urine of a patient with neuroinvasive disease and high WNV RNA load in urine.
Received February 23, 2013. - Revision received March 18, 2013. Accepted March 20, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
-Luisa Barzon 1,2, Monia Pacenti 2, Elisa Franchin 1,2, Silvana Pagni 1,2, Thomas Martello 2, Margherita Cattai 2, Riccardo Cusinato 2 and Giorgio Pal? 1,2
Author Affiliations: <SUP>1</SUP>Department of Molecular Medicine, University of Padova, I-35121 Padova, Italy <SUP>2</SUP>Regional Reference Laboratory for Infectious Diseases, Microbiology and Virology Unit, Padova University Hospital, I-35128 Padova, Italy
Corresponding authors contact information: Luisa Barzon, MD, Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, I-35121 Padova, Italy. Tel. +39-0498218946; Fax +39-0498272355; Email luisa.barzon@unipd.it; Giorgio Pal?, MD, Department of Molecular Medicine, University of Padova, Via A. Gabelli 63, I-35121 Padova, Italy. Tel. +39-0498272350; Fax +39-0498272355; Email giorgio.palu@unipd.it
Abstract
Detection of West Nile virus (WNV) RNA in urine has been anecdotally described and proposed for the diagnosis of WNV infection. This study reports the application of real-time RT-PCR for the detection of WNV RNA in urine as a routine procedure in support to diagnosis of WNV infection during the large outbreak that occurred in North-Eastern Italy in 2012. Fourteen out of 32 (43.8%) patients with symptomatic WNV infection, i.e., neuroinvasive disease and fever, had detectable WNV RNA in urine at the time of diagnosis, at a higher rate and load and for longer time than detection of WNV RNA in blood. At variance, detection of WNV RNA in urine was less frequent (2 out of 14, 14.2%) in blood donors in whom WNV infection was identified by WNV NAAT screening. Infectious virus was isolated from urine of a patient with neuroinvasive disease and high WNV RNA load in urine.
Received February 23, 2013. - Revision received March 18, 2013. Accepted March 20, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
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