tetano
Editor, Senior Moderator
J Immunol
. 2021 May 12;ji2000922.
doi: 10.4049/jimmunol.2000922. Online ahead of print.
Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques
Xiaojuan Liu[SUP] 1 2 [/SUP], Yuzhong Li[SUP] 1 2 [/SUP], Hongjian Xiao[SUP] 1 2 [/SUP], Yanwei Bi[SUP] 1 2 [/SUP], Yue Gong[SUP] 1 2 [/SUP], Zhengrong R Hu[SUP] 1 2 [/SUP], Yaxin Zeng[SUP] 1 2 [/SUP], Ming Sun[SUP] 1 2 [/SUP], Zhanlong L He[SUP] 1 2 [/SUP], Shan Lu[SUP] 3 [/SUP], Qihan Li[SUP] 1 2 [/SUP], Wei Cun[SUP] 4 2 [/SUP]
Affiliations
Abstract
The T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. The present study was undertaken to determine the T cell epitopes in the spike (S) protein of SARS-CoV-2 that dominate the T cell responses in SARS-CoV-2-infected patients. PBMCs from rhesus macaques vaccinated with a DNA vaccine encoding the full-length S protein were isolated, and an ELISPOT assay was used to identify the recognized T cell epitopes among a total of 158 18-mer and 10-aa-overlapping peptides spanning the full-length S protein. Six multipeptide-based epitopes located in the S1 region, with four of the six located in the receptor-binding domain, were defined as the most frequently recognized epitopes in macaques. The conservation of the epitopes across species was also verified, and peptide mixtures for T cell response detection were established. Six newly defined T cell epitopes were found in the current study, which may provide a novel potential target for T cell response detection and the diagnosis and vaccine design of SARS-CoV-2 based on multipeptide subunit-based epitopes.
. 2021 May 12;ji2000922.
doi: 10.4049/jimmunol.2000922. Online ahead of print.
Identification of T Cell Epitopes in the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus 2 in Rhesus Macaques
Xiaojuan Liu[SUP] 1 2 [/SUP], Yuzhong Li[SUP] 1 2 [/SUP], Hongjian Xiao[SUP] 1 2 [/SUP], Yanwei Bi[SUP] 1 2 [/SUP], Yue Gong[SUP] 1 2 [/SUP], Zhengrong R Hu[SUP] 1 2 [/SUP], Yaxin Zeng[SUP] 1 2 [/SUP], Ming Sun[SUP] 1 2 [/SUP], Zhanlong L He[SUP] 1 2 [/SUP], Shan Lu[SUP] 3 [/SUP], Qihan Li[SUP] 1 2 [/SUP], Wei Cun[SUP] 4 2 [/SUP]
Affiliations
- PMID: 33980582
- DOI: 10.4049/jimmunol.2000922
Abstract
The T cell response is an important detection index in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development. The present study was undertaken to determine the T cell epitopes in the spike (S) protein of SARS-CoV-2 that dominate the T cell responses in SARS-CoV-2-infected patients. PBMCs from rhesus macaques vaccinated with a DNA vaccine encoding the full-length S protein were isolated, and an ELISPOT assay was used to identify the recognized T cell epitopes among a total of 158 18-mer and 10-aa-overlapping peptides spanning the full-length S protein. Six multipeptide-based epitopes located in the S1 region, with four of the six located in the receptor-binding domain, were defined as the most frequently recognized epitopes in macaques. The conservation of the epitopes across species was also verified, and peptide mixtures for T cell response detection were established. Six newly defined T cell epitopes were found in the current study, which may provide a novel potential target for T cell response detection and the diagnosis and vaccine design of SARS-CoV-2 based on multipeptide subunit-based epitopes.