J Gen Virol. Pathogenicity of highly pathogenic avian H5N1 influenza A viruses isolated from humans between 2003 and 2008 in northern Vietnam

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Originally published as JGV in Press, 10.1099/vir.0.021659-0 on June 30, 2010 [SIZE=-1]J Gen Virol 91 (2010), 2485-2490; DOI 10.1099/vir.0.021659-0[/SIZE]

Short Communication

Pathogenicity of highly pathogenic avian H5N1 influenza A viruses isolated from humans between 2003 and 2008 in northern Vietnam

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</td></tr></tbody></table><nobr>Quynh Mai Le¹</nobr>, <nobr>Mutsumi Ito²</nobr>, <nobr>Yukiko Muramoto²^,3</nobr>, <nobr>Phuong Vu Mai Hoang¹</nobr>, <nobr>Cuong Duc Vuong¹</nobr>, <nobr>Yuko Sakai-Tagawa²</nobr>, <nobr>Maki Kiso²</nobr>, <nobr>Makoto Ozawa⁴^,5</nobr>, <nobr>Ryo Takano²^,3</nobr> and <nobr>Yoshihiro Kawaoka²^,3^,4^,5^,6</nobr>
[SIZE=-1] ¹ National Institute of Hygiene and Epidemiology (NIHE), Hanoi, Vietnam
² Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
³ ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012, Japan
⁴ International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
⁵ Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA
⁶ Division of Zoonosis, Department of Microbiology and Infectious Diseases, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan [/SIZE]

[SIZE=-1]Correspondence
Yoshihiro Kawaoka
kawaoka@ims.u-tokyo.ac.jp[/SIZE]

Vietnam is one of the countries most affected by highly pathogenicH5N1 influenza A viruses. To evaluate the potential pathogenicityin mammals of H5N1 viruses isolated from humans in Vietnam,we determined the sequences of all eight genes of 22 human isolatescollected between 2003 and 2008 and compared their virulencein mice. The isolates were classified into clade 1 and clade2.3.4 and differed in pathogenicity for mice. Whilst lysineat position 627 of PB2 (PB2-627K) is a critical virulence determinantfor clade 2.3.4 viruses, asparagine at position 701 of PB2 andother unknown virulence determinants appear to be involved inthe high pathogenicity of clade 1 viruses, warranting furtherstudies to determine the factors responsible for the high virulenceof H5N1 viruses in mammals.
The GenBank/EMBL/DDBJ accession numbers for the sequences obtainedin this study are HM114446 [GenBank] ?HM114621.


http://vir.sgmjournals.org/cgi/conte...act/91/10/2485