tetano
Editor, Senior Moderator
J Control Release
. 2023 Nov 28:S0168-3659(23)00764-2.
doi: 10.1016/j.jconrel.2023.11.047. Online ahead of print. A self-assembled graphene oxide adjuvant induces both enhanced humoral and cellular immune responses in influenza vaccine
Shiyi Huang[SUP] 1 [/SUP], Yiyang Li[SUP] 2 [/SUP], Shuang Zhang[SUP] 2 [/SUP], Youming Chen[SUP] 2 [/SUP], Wenqiong Su[SUP] 2 [/SUP], David J Sanchez[SUP] 3 [/SUP], John D H Mai[SUP] 4 [/SUP], Xiao Zhi[SUP] 5 [/SUP], Hongjun Chen[SUP] 6 [/SUP], Xianting Ding[SUP] 7 [/SUP]
Affiliations
Antiviral vaccine is essential for preventing and controlling virus spreading, along with declining morbidity and mortality. A major challenge in effective vaccination lies in the ability to enhance both the humoral and cellular immune responses by adjuvants. Herein, self-assembled nanoparticles based on graphene oxide quantum dots with components of carnosine, resiquimod and Zn[SUP]2+[/SUP] ions, namely ZnGC-R, are designed as a new adjuvant for influenza vaccine. With its high capability for antigen-loading, ZnGC-R enhances antigen utilization, improves DC recruitment, and activates antigen-presenting cells. Single cell analysis of lymphocytes after intramuscular vaccination revealed that ZnGC-R generated multifaceted immune responses. ZnGC-R stimulated robust CD4[SUP]+[/SUP]CCR7[SUP]lo[/SUP]PD-1[SUP]hi[/SUP] Tfh and durable CD8[SUP]+[/SUP]CD44[SUP]hi[/SUP]CD62L[SUP]-[/SUP] T[SUB]EM[/SUB] immune responses, and simultaneously promoted the proliferation of CD26[SUP]+[/SUP] germinal center B cells. Besides, ZnGC-R elicited 2.53-fold higher hemagglutination-inhibiting antibody than commercial-licensed aluminum salt adjuvant. ZnGC-R based vaccine induced 342% stronger IgG antibody responses compared with vaccines with inactivated virus alone, leading to 100% in vivo protection efficacy against the H1N1 influenza virus challenge.
Keywords: Adjuvant; Graphene oxide quantum dots; Influenza; Mass cytometry; Vaccine.
. 2023 Nov 28:S0168-3659(23)00764-2.
doi: 10.1016/j.jconrel.2023.11.047. Online ahead of print. A self-assembled graphene oxide adjuvant induces both enhanced humoral and cellular immune responses in influenza vaccine
Shiyi Huang[SUP] 1 [/SUP], Yiyang Li[SUP] 2 [/SUP], Shuang Zhang[SUP] 2 [/SUP], Youming Chen[SUP] 2 [/SUP], Wenqiong Su[SUP] 2 [/SUP], David J Sanchez[SUP] 3 [/SUP], John D H Mai[SUP] 4 [/SUP], Xiao Zhi[SUP] 5 [/SUP], Hongjun Chen[SUP] 6 [/SUP], Xianting Ding[SUP] 7 [/SUP]
Affiliations
- PMID: 38036004
- DOI: 10.1016/j.jconrel.2023.11.047
Antiviral vaccine is essential for preventing and controlling virus spreading, along with declining morbidity and mortality. A major challenge in effective vaccination lies in the ability to enhance both the humoral and cellular immune responses by adjuvants. Herein, self-assembled nanoparticles based on graphene oxide quantum dots with components of carnosine, resiquimod and Zn[SUP]2+[/SUP] ions, namely ZnGC-R, are designed as a new adjuvant for influenza vaccine. With its high capability for antigen-loading, ZnGC-R enhances antigen utilization, improves DC recruitment, and activates antigen-presenting cells. Single cell analysis of lymphocytes after intramuscular vaccination revealed that ZnGC-R generated multifaceted immune responses. ZnGC-R stimulated robust CD4[SUP]+[/SUP]CCR7[SUP]lo[/SUP]PD-1[SUP]hi[/SUP] Tfh and durable CD8[SUP]+[/SUP]CD44[SUP]hi[/SUP]CD62L[SUP]-[/SUP] T[SUB]EM[/SUB] immune responses, and simultaneously promoted the proliferation of CD26[SUP]+[/SUP] germinal center B cells. Besides, ZnGC-R elicited 2.53-fold higher hemagglutination-inhibiting antibody than commercial-licensed aluminum salt adjuvant. ZnGC-R based vaccine induced 342% stronger IgG antibody responses compared with vaccines with inactivated virus alone, leading to 100% in vivo protection efficacy against the H1N1 influenza virus challenge.
Keywords: Adjuvant; Graphene oxide quantum dots; Influenza; Mass cytometry; Vaccine.