tetano
Editor, Senior Moderator
J Clin Virol. 2020 May 7;128:104423. doi: 10.1016/j.jcv.2020.104423. [Epub ahead of print]
SARS-CoV-2 detection by direct rRT-PCR without RNA extraction.
Merindol N[SUP]1[/SUP], P?pin G[SUP]2[/SUP], Marchand C[SUP]3[/SUP], Rheault M[SUP]4[/SUP], Peterson C[SUP]5[/SUP], Poirier A[SUP]6[/SUP], Houle C[SUP]7[/SUP], Germain H[SUP]8[/SUP], Danylo A[SUP]9[/SUP].
Author information
Abstract
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar? SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.
Copyright ? 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
KEYWORDS:
COVID19; Coronavirus; Direct rRTPCR; RNA extraction; SARS-CoV-2; Virus detection
PMID:32416598DOI:10.1016/j.jcv.2020.104423
SARS-CoV-2 detection by direct rRT-PCR without RNA extraction.
Merindol N[SUP]1[/SUP], P?pin G[SUP]2[/SUP], Marchand C[SUP]3[/SUP], Rheault M[SUP]4[/SUP], Peterson C[SUP]5[/SUP], Poirier A[SUP]6[/SUP], Houle C[SUP]7[/SUP], Germain H[SUP]8[/SUP], Danylo A[SUP]9[/SUP].
Author information
Abstract
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar? SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.
Copyright ? 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
KEYWORDS:
COVID19; Coronavirus; Direct rRTPCR; RNA extraction; SARS-CoV-2; Virus detection
PMID:32416598DOI:10.1016/j.jcv.2020.104423