Giuseppe
Emeritus
[Source: US National Library of Medicine, full text: (LINK): Abstract, edited.]
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J Clin Virol. 2013 May 9. pii: S1386-6532(13)00122-4. doi: 10.1016/j.jcv.2013.04.002. [Epub ahead of print]
Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses.
Wang C, Sun X, Suo S, Ren Y, Li X, Herrler G, Thiel V, Ren X.
Source: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Abstract
BACKGROUND:
Transmission of SARS-associated coronavirus (SARS-CoV) is now well controlled, nevertheless, it is important to develop effective methods to identify this virus from other pathogens.
OBJECTIVES:
The purpose of this study was to identify potential ligands and develop a novel diagnostic test to SARS-CoV using phage display technology.
STUDY DESIGN:
The SARS-CoV spike 1 (S1) protein containing the receptor binding region (RBD) was used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After four rounds of biopanning, 10 monoclonal phages with specific binding activity to the S1-RBD protein were obtained and subjected to binding and diagnostic assays.
RESULTS:
DNA sequencing showed that two phage displayed peptides HHKTWHPPVMHL (phage-H) and SQWHPRSASYPM (phage-S) that were specific ligands to the S1 protein. Moreover, the selected phage-H and phage-S were capable of differentiating SARS-CoV from other coronaviruses in indirect enzyme-linked immunosorbent assays.
CONCLUSION:
The peptides identified in this study are useful reagents for detection of SARS-CoV.
Copyright ? 2013 Elsevier B.V. All rights reserved.
PMID: 23664850 [PubMed - as supplied by publisher]
-Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses.
Wang C, Sun X, Suo S, Ren Y, Li X, Herrler G, Thiel V, Ren X.
Source: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Abstract
BACKGROUND:
Transmission of SARS-associated coronavirus (SARS-CoV) is now well controlled, nevertheless, it is important to develop effective methods to identify this virus from other pathogens.
OBJECTIVES:
The purpose of this study was to identify potential ligands and develop a novel diagnostic test to SARS-CoV using phage display technology.
STUDY DESIGN:
The SARS-CoV spike 1 (S1) protein containing the receptor binding region (RBD) was used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After four rounds of biopanning, 10 monoclonal phages with specific binding activity to the S1-RBD protein were obtained and subjected to binding and diagnostic assays.
RESULTS:
DNA sequencing showed that two phage displayed peptides HHKTWHPPVMHL (phage-H) and SQWHPRSASYPM (phage-S) that were specific ligands to the S1 protein. Moreover, the selected phage-H and phage-S were capable of differentiating SARS-CoV from other coronaviruses in indirect enzyme-linked immunosorbent assays.
CONCLUSION:
The peptides identified in this study are useful reagents for detection of SARS-CoV.
Copyright ? 2013 Elsevier B.V. All rights reserved.
PMID: 23664850 [PubMed - as supplied by publisher]
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