tetano
Editor, Senior Moderator
J Clin Virol
. 2021 Oct 9;144:104998.
doi: 10.1016/j.jcv.2021.104998. Online ahead of print.
Diagnostic performance of the Idylla™ respiratory panel for molecular detection of influenza A/B in patients presenting to primary care with influenza-like illness during 3 consecutive influenza seasons
Veerle Matheeussen[SUP] 1 [/SUP], Katherine Loens[SUP] 2 [/SUP], Mandy Kuijstermans[SUP] 3 [/SUP], Kevin Jacobs[SUP] 3 [/SUP], Samuel Coenen[SUP] 3 [/SUP], Alike W van der Velden[SUP] 4 [/SUP], Emily Bongard[SUP] 5 [/SUP], Chris C Butler[SUP] 5 [/SUP], Theo Jm Verheij[SUP] 4 [/SUP], Herman Goossens[SUP] 2 [/SUP], Margareta Ieven[SUP] 2 [/SUP]
Affiliations
Abstract
Background: Influenza virus (IFV) is often encountered in primary care. Implementation of a rapid diagnostic test for its detection at the point-of-care would enable discrimination from other viral causes of influenza-like-illness (ILI) and might be helpful in individual patient management. In this study, the diagnostic performance of such a point-of-care platform was evaluated.
Methods: Respiratory samples (n = 1490) from ILI-patients in primary care in 15 European countries were collected as part of a prospective clinical trial. Both children (n = 252) and adults (n = 1238) were sampled during 3 consecutive periods of high IFV endemicity. Samples were analysed in a central laboratory, after storage at -70 °C, with the Idylla™ Respiratory Panel, detecting both IFV and RSV, on the Idylla™ platform. The Fast Track Diagnostics (FTD) Respiratory Pathogens 21 plus assay was used as reference. A subset of samples (n = 192) was analysed both fresh and after being frozen.
Results: The reference method detected IFV-A in 42% and IFV-B in 13% of the samples. Sensitivity of the Idylla for detection of IFV-A and IFV-B was 98.2% and 92.3% and specificity 97.7% and 98.4% respectively. False negative samples contained significantly lower viral loads than true positive samples (FTD mean Ct-value 30.7 versus 26.1 for IFV-A and 30.4 versus 25.1 for IFV-B, p < 0.001). Comparable results were obtained for Idylla analysis using fresh and frozen samples.
Conclusions: The Idylla Respiratory Panel is a promising point-of-care test for detection of IFV in ILI patients due to its excellent diagnostic performance, minimal training requirements and limited hands-on time.
Keywords: Point of care test; Rapid diagnostic test; Respiratory viruses.
. 2021 Oct 9;144:104998.
doi: 10.1016/j.jcv.2021.104998. Online ahead of print.
Diagnostic performance of the Idylla™ respiratory panel for molecular detection of influenza A/B in patients presenting to primary care with influenza-like illness during 3 consecutive influenza seasons
Veerle Matheeussen[SUP] 1 [/SUP], Katherine Loens[SUP] 2 [/SUP], Mandy Kuijstermans[SUP] 3 [/SUP], Kevin Jacobs[SUP] 3 [/SUP], Samuel Coenen[SUP] 3 [/SUP], Alike W van der Velden[SUP] 4 [/SUP], Emily Bongard[SUP] 5 [/SUP], Chris C Butler[SUP] 5 [/SUP], Theo Jm Verheij[SUP] 4 [/SUP], Herman Goossens[SUP] 2 [/SUP], Margareta Ieven[SUP] 2 [/SUP]
Affiliations
- PMID: 34653942
- DOI: 10.1016/j.jcv.2021.104998
Abstract
Background: Influenza virus (IFV) is often encountered in primary care. Implementation of a rapid diagnostic test for its detection at the point-of-care would enable discrimination from other viral causes of influenza-like-illness (ILI) and might be helpful in individual patient management. In this study, the diagnostic performance of such a point-of-care platform was evaluated.
Methods: Respiratory samples (n = 1490) from ILI-patients in primary care in 15 European countries were collected as part of a prospective clinical trial. Both children (n = 252) and adults (n = 1238) were sampled during 3 consecutive periods of high IFV endemicity. Samples were analysed in a central laboratory, after storage at -70 °C, with the Idylla™ Respiratory Panel, detecting both IFV and RSV, on the Idylla™ platform. The Fast Track Diagnostics (FTD) Respiratory Pathogens 21 plus assay was used as reference. A subset of samples (n = 192) was analysed both fresh and after being frozen.
Results: The reference method detected IFV-A in 42% and IFV-B in 13% of the samples. Sensitivity of the Idylla for detection of IFV-A and IFV-B was 98.2% and 92.3% and specificity 97.7% and 98.4% respectively. False negative samples contained significantly lower viral loads than true positive samples (FTD mean Ct-value 30.7 versus 26.1 for IFV-A and 30.4 versus 25.1 for IFV-B, p < 0.001). Comparable results were obtained for Idylla analysis using fresh and frozen samples.
Conclusions: The Idylla Respiratory Panel is a promising point-of-care test for detection of IFV in ILI patients due to its excellent diagnostic performance, minimal training requirements and limited hands-on time.
Keywords: Point of care test; Rapid diagnostic test; Respiratory viruses.