Giuseppe
Emeritus
J Clin Microbiol. 2009 Aug 5. [Epub ahead of print]
Genetic Evolution of H9 Subtype Influenza Viruses from the live poultry markets of Shanghai in China.
Ge FF, Zhou JP, Liu J, Wang J, Zhang WY, Sheng LP, Xv F, Ju HB, Sun QY, Liu PH. - Shanghai Animal Disease Control Center, Shanghai 201103, P R China.
H9N2 influenza viruses have become established and maintain long-term endemicity in poultry. The complete genome of seven avian H9N2 influenza viruses were characterized. These seven influenza isolates were obtained from live poultry markets of Shanghai in China from 2002, 2006 to 2008. Genetic analysis revealed that all seven isolates had an RSSR motif at the cleavage site of HA, representing low pathogenicity in chickens. Phylogenetic analyses indicated that the seven avian H9N2 viruses belonged to the lineage represented by Duck/Hong Kong/Y280/97 (H9N2), a virus belonging to the Chicken/Beijing/1/94-like (H9N2) lineage and they are all quadruple reassortants consisting of genes from different lineage. The six internal genes of the isolates possessed H5N1-like sequences, indicating that they were reassortants of H9 and H5 viruses. All of the viruses had nonstructural (and HA, NA) genes derived from the Duck/Hong Kong/Y280/97-like virus lineage but had other genes of mixed avian virus origin, including genes similar to those of H5N1 viruses (Gs/GD like). The infected chickens showed no signs of disease. These results show genetic and biologic diversity of H9N2 viruses in Shanghai and support their potential role as pandemic influenza agents.
PMID: 19656985 [PubMed - as supplied by publishe
-
------
Genetic Evolution of H9 Subtype Influenza Viruses from the live poultry markets of Shanghai in China.
Ge FF, Zhou JP, Liu J, Wang J, Zhang WY, Sheng LP, Xv F, Ju HB, Sun QY, Liu PH. - Shanghai Animal Disease Control Center, Shanghai 201103, P R China.
H9N2 influenza viruses have become established and maintain long-term endemicity in poultry. The complete genome of seven avian H9N2 influenza viruses were characterized. These seven influenza isolates were obtained from live poultry markets of Shanghai in China from 2002, 2006 to 2008. Genetic analysis revealed that all seven isolates had an RSSR motif at the cleavage site of HA, representing low pathogenicity in chickens. Phylogenetic analyses indicated that the seven avian H9N2 viruses belonged to the lineage represented by Duck/Hong Kong/Y280/97 (H9N2), a virus belonging to the Chicken/Beijing/1/94-like (H9N2) lineage and they are all quadruple reassortants consisting of genes from different lineage. The six internal genes of the isolates possessed H5N1-like sequences, indicating that they were reassortants of H9 and H5 viruses. All of the viruses had nonstructural (and HA, NA) genes derived from the Duck/Hong Kong/Y280/97-like virus lineage but had other genes of mixed avian virus origin, including genes similar to those of H5N1 viruses (Gs/GD like). The infected chickens showed no signs of disease. These results show genetic and biologic diversity of H9N2 viruses in Shanghai and support their potential role as pandemic influenza agents.
PMID: 19656985 [PubMed - as supplied by publishe
-
------