Giuseppe
Emeritus
Development and preliminary evaluation of a rapid oligochromatographic assay for the specific detection of new human influenza A H1N1 virus. (J Clin Microbiol., abstract, edited)
[Source: US National Library of Medicine, LINK. EDITED.]
J Clin Microbiol. 2010 Mar 10. [Epub ahead of print]
Development and preliminary evaluation of a rapid oligochromatographic assay for the specific detection of new human influenza A H1N1 virus.
P?rez-Ruiz M, Navarro-Mar? JM, Bautista-Mar?n MF, Pedrosa-Corral I, Sanbonmatsu-G?mez S, Camacho AG, Rojas J, Ruiz-Ortiz J, Rodr?guez-Granger J,Carrillo JA. - Servicio de Microbiolog?a, Hospital Universitario Virgen de las Nieves; Vircell, S.L. Pol?gono Industrial Dos de Octubre. Plaza Dom?nguez Ortiz; Santa F?. 18320. Granada, Spain.
A new oligochromatographic assay, SPEED-OLIGO NOVEL INFLUENZA A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hemagglutinin gene. The analytical sensitivity of the new assay was measured with serial dilutions of a plasmid that contained the target sequence, finding that down to one copy per reaction of the plasmid was reliably detected. Diagnostic performance was assessed with 103 RNAs from suspected cases (40 positive and 63 negative results) previously analyzed with a reference real-time PCR technique. All positive cases were confirmed and no false-positive results were detected with the new assay. No cross-reactions were observed when other viral strains or clinical samples with other respiratory viruses were tested. According to the seresults, this new assay has 100% sensitivity and specificity. The turnaround time for the whole procedure was 140 min. The assay may be especially useful for the specific detection of 2009 H1N1virus in laboratories not equipped with real-time PCR instruments.
PMID: 20220170 [PubMed - as supplied by publisher]
J Clin Microbiol. 2010 Mar 10. [Epub ahead of print]
Development and preliminary evaluation of a rapid oligochromatographic assay for the specific detection of new human influenza A H1N1 virus.
P?rez-Ruiz M, Navarro-Mar? JM, Bautista-Mar?n MF, Pedrosa-Corral I, Sanbonmatsu-G?mez S, Camacho AG, Rojas J, Ruiz-Ortiz J, Rodr?guez-Granger J,Carrillo JA. - Servicio de Microbiolog?a, Hospital Universitario Virgen de las Nieves; Vircell, S.L. Pol?gono Industrial Dos de Octubre. Plaza Dom?nguez Ortiz; Santa F?. 18320. Granada, Spain.
A new oligochromatographic assay, SPEED-OLIGO NOVEL INFLUENZA A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hemagglutinin gene. The analytical sensitivity of the new assay was measured with serial dilutions of a plasmid that contained the target sequence, finding that down to one copy per reaction of the plasmid was reliably detected. Diagnostic performance was assessed with 103 RNAs from suspected cases (40 positive and 63 negative results) previously analyzed with a reference real-time PCR technique. All positive cases were confirmed and no false-positive results were detected with the new assay. No cross-reactions were observed when other viral strains or clinical samples with other respiratory viruses were tested. According to the seresults, this new assay has 100% sensitivity and specificity. The turnaround time for the whole procedure was 140 min. The assay may be especially useful for the specific detection of 2009 H1N1virus in laboratories not equipped with real-time PCR instruments.
PMID: 20220170 [PubMed - as supplied by publisher]