Giuseppe
Emeritus
Comparison of polyurethane foam vs. nylon flocked swab collection of anterior nares secretions on performance of a rapid influenza virus antigen test in a pediatric emergency department. (J Clin Microbiol, abstract, edited, RA-1003)
J Clin Microbiol. 2010 Jan 6. [Epub ahead of print]
Comparison of polyurethane foam vs. nylon flocked swab collection of anterior nares secretions on performance of a rapid influenza virus antigen test in a pediatric emergency department.
Scansen KA, Bonsu BK, Stoner E, Mack K, Salamon D, Leber A, Marcon MJ. - Departments of Pediatrics and Pathology, The Ohio State University College of Medicine, and Departments of Emergency Medicine and Laboratory Medicine, Nationwide Children's Hospital, Columbus, Ohio 43205.
Rapid antigen testing of upper respiratory secretions collected with various swab types is often utilized for laboratory diagnosis of influenza virus infection. There are limited data on the effects of swab composition on test performance. This study compared the performance of the Quidel QuickVue influenza A+B test on anterior nares secretions when a polyurethane foam swab versus a nylon flocked swab was used for collection. One hundred subjects who presented to a pediatric emergency department with symptoms suggestive of an influenza infection were recruited for the study. Foam and flocked anterior nares swabs were obtained from separate nares on each subject before a posterior nasopharyngeal swab was collected and placed into viral transport medium. The QuickVue test was performed directly on each swab type and the results compared to reverse transcription-polymerase chain reaction (RT-PCR), direct fluorescent antibody (DFA), and viral culture performed on the transport medium. RT-PCR alone or DFA combined with culture were utilized as separate gold standards. There were 56 cases of influenza detected by RT-PCR; the QuickVue test was positive on 40 foam and 30 flocked swabs for a sensitivity of 71% and 53%, respectively (p=0.01). Similarly, there were 49 influenza cases detected by DFA and/or culture; the QuickVue test was positive on 38 foam and 30 flocked swabs for a sensitivity of 78% and 61%, respectively (p=0.013). This study suggests that anterior nares collections using polyurethane foam swabs perform better than nylon flocked swabs in the Quidel QuickVue influenza A+B test.
PMID: 20053857 [PubMed - as supplied by publisher]
-
------
J Clin Microbiol. 2010 Jan 6. [Epub ahead of print]
Comparison of polyurethane foam vs. nylon flocked swab collection of anterior nares secretions on performance of a rapid influenza virus antigen test in a pediatric emergency department.
Scansen KA, Bonsu BK, Stoner E, Mack K, Salamon D, Leber A, Marcon MJ. - Departments of Pediatrics and Pathology, The Ohio State University College of Medicine, and Departments of Emergency Medicine and Laboratory Medicine, Nationwide Children's Hospital, Columbus, Ohio 43205.
Rapid antigen testing of upper respiratory secretions collected with various swab types is often utilized for laboratory diagnosis of influenza virus infection. There are limited data on the effects of swab composition on test performance. This study compared the performance of the Quidel QuickVue influenza A+B test on anterior nares secretions when a polyurethane foam swab versus a nylon flocked swab was used for collection. One hundred subjects who presented to a pediatric emergency department with symptoms suggestive of an influenza infection were recruited for the study. Foam and flocked anterior nares swabs were obtained from separate nares on each subject before a posterior nasopharyngeal swab was collected and placed into viral transport medium. The QuickVue test was performed directly on each swab type and the results compared to reverse transcription-polymerase chain reaction (RT-PCR), direct fluorescent antibody (DFA), and viral culture performed on the transport medium. RT-PCR alone or DFA combined with culture were utilized as separate gold standards. There were 56 cases of influenza detected by RT-PCR; the QuickVue test was positive on 40 foam and 30 flocked swabs for a sensitivity of 71% and 53%, respectively (p=0.01). Similarly, there were 49 influenza cases detected by DFA and/or culture; the QuickVue test was positive on 38 foam and 30 flocked swabs for a sensitivity of 78% and 61%, respectively (p=0.013). This study suggests that anterior nares collections using polyurethane foam swabs perform better than nylon flocked swabs in the Quidel QuickVue influenza A+B test.
PMID: 20053857 [PubMed - as supplied by publisher]
-
------