Giuseppe
Emeritus
J Clin Microbiol. 2008 Dec 17. [Epub ahead of print]
Co-circulation of Antigenic Variants and the Vaccine-type Virus During the 2004/2005 Influenza B Virus Epidemics in Japan.
Nakagawa N, Higashi N, Nakagawa T. - Department of Microbiology, Kobe Institute of Health, 4-6, Minatojima-nakamachi, Chuo-ku, Kobe, 650-0046 Japan; Department of Infectious Control, Osaka Medical College Hospital, 2-7 Daigaku-cho Takatsuki 569-8686 Japan.
In the 2004/2005 season, there was a large epidemic of the influenza B virus Yamagata group in Kobe, Japan.
On the hemagglutination inhibition tests, most of the clinical isolates in Kobe showed similar antigenicities to those of previous isolates (the vaccine-type virus).
Only a few antigenic variants were isolated around the peak of the epidemic; however, Kobe residents developed antibodies against the variants during the season.
The antigenic variants showed a one-point mutation of a nucleotide in the HA1 gene (C440A or G421A), which resulted in the substitution of one amino acid in the 150 loop of the HA molecule (T147N or G141R).
The 150 loop is one of 4 epitopes of the hemagglutinin molecule of the influenza B virus.
We established a system to detect one-point differences in the nucleotides of the 150 loop by means of high-resolution melting curve analysis with LCGreen.
With this system, the isolates were determined to be the vaccine-type virus, antigenic variants or a mixture of both.
Some isolates were shown to be mixtures, although they had been recognized as the vaccine-type virus with the hemagglutination inhibition tests.
Thus, the antigenic variants appeared in the early period of the epidemic and were co-circulating with the vaccine-type virus during the epidemic.
PMID: 19091818 [PubMed - as supplied by publisher]
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Co-circulation of Antigenic Variants and the Vaccine-type Virus During the 2004/2005 Influenza B Virus Epidemics in Japan.
Nakagawa N, Higashi N, Nakagawa T. - Department of Microbiology, Kobe Institute of Health, 4-6, Minatojima-nakamachi, Chuo-ku, Kobe, 650-0046 Japan; Department of Infectious Control, Osaka Medical College Hospital, 2-7 Daigaku-cho Takatsuki 569-8686 Japan.
In the 2004/2005 season, there was a large epidemic of the influenza B virus Yamagata group in Kobe, Japan.
On the hemagglutination inhibition tests, most of the clinical isolates in Kobe showed similar antigenicities to those of previous isolates (the vaccine-type virus).
Only a few antigenic variants were isolated around the peak of the epidemic; however, Kobe residents developed antibodies against the variants during the season.
The antigenic variants showed a one-point mutation of a nucleotide in the HA1 gene (C440A or G421A), which resulted in the substitution of one amino acid in the 150 loop of the HA molecule (T147N or G141R).
The 150 loop is one of 4 epitopes of the hemagglutinin molecule of the influenza B virus.
We established a system to detect one-point differences in the nucleotides of the 150 loop by means of high-resolution melting curve analysis with LCGreen.
With this system, the isolates were determined to be the vaccine-type virus, antigenic variants or a mixture of both.
Some isolates were shown to be mixtures, although they had been recognized as the vaccine-type virus with the hemagglutination inhibition tests.
Thus, the antigenic variants appeared in the early period of the epidemic and were co-circulating with the vaccine-type virus during the epidemic.
PMID: 19091818 [PubMed - as supplied by publisher]
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