isolation of H5N1 in human feces

[Anne]

seems to me there were feces isolations in some patients in vietnam?



http://www.sciencedirect.com/scienc...serid=10&md5=9dbee026f72989cc5d967ed725697915


Influenza A/H5N1 virus infection in humans in Cambodia

Philippe Buchy^a^,^,, Sek Mardy^a, Sirenda Vong^a, Tetsuya Toyoda^b, Jean-Thierry Aubin^c, Megge Miller^d, Sok Touch^e, Ly Sovann^e, Jean-Baptiste Dufourcq^f, Beat Richner^g, Phan Van Tu^h, Nguyen Thi Kim Tien^h, Wilina Limⁱ, J.S. Malik Peiris^j and Sylvie Van der Werf^c
^aInstitut Pasteur in Cambodia, 5 Monivong blvd, P.O. Box 983, Phnom Penh, Cambodia
^bInstitut Pasteur of Shanghai, 225 South Chonqing Road, Shanghai 200025, China
^cInstitut Pasteur, Unit? de Biologie Mol?culaire des Virus Respiratoires, 25-28 rue du Dr Roux, Paris, France
^dWorld Health Organization, 51 Pasteur Street, Phnom Penh, Cambodia
^eMinistry of Health, Communicable Disease Department, Phnom Penh, Cambodia
^fCalmette Hospital, Intensive Care Unit, Monivong blvd, Phnom Penh, Cambodia
^gKantha Bopha Hospitals Foundation, Cambodia
^hInstitut Pasteur in Ho Chi Minh City, 167 Pasteur Street, Ho Chi Minh City, Vietnam
ⁱHong Kong Department of Health, Hong Kong Special Administrative Region, People's Republic of China
^jHKU-Pasteur Research Centre and Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China
Received 22 January 2007; accepted 16 April 2007. Available online 29 May 2007.


Abstract

Background

Between January 2005 and April 2006, six patients of influenza A/H5N1 virus infection were reported in Cambodia, all with fatal outcome.
Objectives

We describe the virological findings of these six H5N1 patients in association with clinical and epidemiologic findings.
Study design

Broncho-alveolar lavage, nasopharyngeal, throat and rectal swabs and sera were cultured for virus isolation and viral load quantified in clinical specimens by real-time RT-PCR. We compared sequences obtained from different body sites within the same patient to detect viral quasi-species.
Results

H5N1 virus strains isolated in Cambodia belong to genotype Z, clade 1 viruses. H5N1 viruses were isolated from serum and rectal swab specimens in two patients. The haemagglutinin gene sequences of the virus in different body sites did not differ. Amino acid substitutions known to be associated with a change in virus binding were not observed.
Conclusion

The high frequency of virus isolation from serum and faecal swabs highlights that H5N1 is likely to be a disseminated infection in humans and this has implications for antiviral treatment, biosafety in clinical laboratories and on risks for nosocomial and human-to-human transmission. There were no tissue-specific adaptive mutations in the HA gene from viruses isolated from different organs.


Keywords: Avian influenza virus; H5N1; Cambodia; Tissue tropism; Virus dissemination