tetano
Editor, Senior Moderator
iScience
. 2024 Dec 18;28(2):111632.
doi: 10.1016/j.isci.2024.111632. eCollection 2025 Feb 21. MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies
Olubukola M Abiona[SUP] 1 [/SUP], Nianshuang Wang[SUP] 2 [/SUP], Sarah R Leist[SUP] 3 [/SUP], Alexandra Schäfer[SUP] 3 [/SUP], Adam S Cockrell[SUP] 3 [/SUP], Lingshu Wang[SUP] 1 [/SUP], Sandhya Bangaru[SUP] 4 [/SUP], Laura Stevens[SUP] 5 [/SUP], Rachel L Graham[SUP] 3 [/SUP], Jacob F Kocher[SUP] 3 [/SUP], Yaroslav Tsybovsky[SUP] 6 [/SUP], Masaru Kanekiyo[SUP] 1 [/SUP], Azad Kumar[SUP] 1 [/SUP], Kaitlyn M Morabito[SUP] 1 [/SUP], Osnat Rosen[SUP] 1 [/SUP], Wei Shi[SUP] 1 [/SUP], Anne Werner[SUP] 1 [/SUP], Yi Zhang[SUP] 1 [/SUP], Cynthia Ziwawo[SUP] 1 [/SUP], Christian K O Dzuvor[SUP] 7 [/SUP], Charis Palandjian[SUP] 7 [/SUP], Connor Eastman[SUP] 8 [/SUP], Hannah R Matthews[SUP] 7 [/SUP], Jeswin Joseph[SUP] 7 [/SUP], James D Chappell[SUP] 5 [/SUP], Wing-Pui Kong[SUP] 1 [/SUP], John R Mascola[SUP] 1 [/SUP], Andrew B Ward[SUP] 4 [/SUP], Mark R Denison[SUP] 5 [/SUP], Ralph Baric[SUP] 3 [/SUP], Jason S McLellan[SUP] 2 [/SUP], Barney S Graham[SUP] 1 [/SUP], Kizzmekia S Corbett-Helaire[SUP] 1 7 9 [/SUP]
Affiliations
The COVID-19 pandemic underscores the need to prepare for future emerging coronavriuses (CoVs) by understanding the principles behind effective CoV vaccine design such as protective immunity and antibody responses. To study which epitopes and subdomains contribute to in vivo protection, we utilized the prefusion-stabilized spike protein of MERS-CoV, MERS S-2P, as a vaccine immunogen. Vaccination with MERS S-2P elicited both receptor-binding domain (RBD)- and non-RBD-specific antibodies, including N-terminal domain (NTD)-specific G2-and CDC2-A2-like antibodies. Intriguingly, the immunogen MERS S-2P_ΔRBD, MERS S-2P with the RBDs removed, protects comparably to S1 and S-2P immunogens against MERS-CoV challenge. Moreover, passive transfer studies of polyclonal IgG from MERS S-2P immunized mice depleted of subdomain-specific antibodies demonstrated that non-RBD antibodies protected more than non-NTD antibodies. Altogether, these findings illustrate that in-vivo protection is not solely driven by RBD-specific antibodies and highlights the importance of targeting non-RBD sites in future CoV vaccine designs.
Keywords: Immunity; Immunology; Virology.
. 2024 Dec 18;28(2):111632.
doi: 10.1016/j.isci.2024.111632. eCollection 2025 Feb 21. MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies
Olubukola M Abiona[SUP] 1 [/SUP], Nianshuang Wang[SUP] 2 [/SUP], Sarah R Leist[SUP] 3 [/SUP], Alexandra Schäfer[SUP] 3 [/SUP], Adam S Cockrell[SUP] 3 [/SUP], Lingshu Wang[SUP] 1 [/SUP], Sandhya Bangaru[SUP] 4 [/SUP], Laura Stevens[SUP] 5 [/SUP], Rachel L Graham[SUP] 3 [/SUP], Jacob F Kocher[SUP] 3 [/SUP], Yaroslav Tsybovsky[SUP] 6 [/SUP], Masaru Kanekiyo[SUP] 1 [/SUP], Azad Kumar[SUP] 1 [/SUP], Kaitlyn M Morabito[SUP] 1 [/SUP], Osnat Rosen[SUP] 1 [/SUP], Wei Shi[SUP] 1 [/SUP], Anne Werner[SUP] 1 [/SUP], Yi Zhang[SUP] 1 [/SUP], Cynthia Ziwawo[SUP] 1 [/SUP], Christian K O Dzuvor[SUP] 7 [/SUP], Charis Palandjian[SUP] 7 [/SUP], Connor Eastman[SUP] 8 [/SUP], Hannah R Matthews[SUP] 7 [/SUP], Jeswin Joseph[SUP] 7 [/SUP], James D Chappell[SUP] 5 [/SUP], Wing-Pui Kong[SUP] 1 [/SUP], John R Mascola[SUP] 1 [/SUP], Andrew B Ward[SUP] 4 [/SUP], Mark R Denison[SUP] 5 [/SUP], Ralph Baric[SUP] 3 [/SUP], Jason S McLellan[SUP] 2 [/SUP], Barney S Graham[SUP] 1 [/SUP], Kizzmekia S Corbett-Helaire[SUP] 1 7 9 [/SUP]
Affiliations
- PMID: 39898019
- PMCID: PMC11783452
- DOI: 10.1016/j.isci.2024.111632
The COVID-19 pandemic underscores the need to prepare for future emerging coronavriuses (CoVs) by understanding the principles behind effective CoV vaccine design such as protective immunity and antibody responses. To study which epitopes and subdomains contribute to in vivo protection, we utilized the prefusion-stabilized spike protein of MERS-CoV, MERS S-2P, as a vaccine immunogen. Vaccination with MERS S-2P elicited both receptor-binding domain (RBD)- and non-RBD-specific antibodies, including N-terminal domain (NTD)-specific G2-and CDC2-A2-like antibodies. Intriguingly, the immunogen MERS S-2P_ΔRBD, MERS S-2P with the RBDs removed, protects comparably to S1 and S-2P immunogens against MERS-CoV challenge. Moreover, passive transfer studies of polyclonal IgG from MERS S-2P immunized mice depleted of subdomain-specific antibodies demonstrated that non-RBD antibodies protected more than non-NTD antibodies. Altogether, these findings illustrate that in-vivo protection is not solely driven by RBD-specific antibodies and highlights the importance of targeting non-RBD sites in future CoV vaccine designs.
Keywords: Immunity; Immunology; Virology.