tetano
Editor, Senior Moderator
iScience
. 2024 Mar 29;27(5):109647.
doi: 10.1016/j.isci.2024.109647. eCollection 2024 May 17. Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection
Tomokazu Tamura[SUP] 1 2 3 [/SUP], Hayato Ito[SUP] 1 [/SUP], Shiho Torii[SUP] 4 [/SUP], Lei Wang[SUP] 5 6 [/SUP], Rigel Suzuki[SUP] 1 2 [/SUP], Shuhei Tsujino[SUP] 1 [/SUP], Akifumi Kamiyama[SUP] 1 [/SUP], Yoshitaka Oda[SUP] 5 [/SUP], Masumi Tsuda[SUP] 5 6 [/SUP], Yuhei Morioka[SUP] 1 4 [/SUP], Saori Suzuki[SUP] 1 2 [/SUP], Kotaro Shirakawa[SUP] 7 [/SUP], Kei Sato[SUP] 8 9 10 11 12 13 14 [/SUP], Kumiko Yoshimatsu[SUP] 15 [/SUP], Yoshiharu Matsuura[SUP] 4 16 [/SUP], Satoshi Iwano[SUP] 17 [/SUP], Shinya Tanaka[SUP] 5 6 [/SUP], Takasuke Fukuhara[SUP] 1 2 3 4 18 [/SUP]
Affiliations
Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome. However, NanoLuc/Furimazine and also the conventional firefly luciferase/D-luciferin are known to exhibit relatively low tissue permeability and thus less sensitivity for visualization of deep tissue including lungs. Here, we demonstrated in vivo sufficient visualization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using the pair of a codon-optimized Akaluc and AkaLumine. We engineered the codon-optimized Akaluc gene possessing the similar GC ratio of SARS-CoV-2. Using the SARS-CoV-2 recombinants carrying the codon-optimized Akaluc, we visualized in vivo infection of respiratory organs, including the tissue-specific differences associated with particular variants. Additionally, we could evaluate the efficacy of antivirals by monitoring changes in Akaluc signals. Overall, we offer an effective technology for monitoring viral dynamics in live animals.
Keywords: Methodology in biological sciences; Virology.
. 2024 Mar 29;27(5):109647.
doi: 10.1016/j.isci.2024.109647. eCollection 2024 May 17. Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection
Tomokazu Tamura[SUP] 1 2 3 [/SUP], Hayato Ito[SUP] 1 [/SUP], Shiho Torii[SUP] 4 [/SUP], Lei Wang[SUP] 5 6 [/SUP], Rigel Suzuki[SUP] 1 2 [/SUP], Shuhei Tsujino[SUP] 1 [/SUP], Akifumi Kamiyama[SUP] 1 [/SUP], Yoshitaka Oda[SUP] 5 [/SUP], Masumi Tsuda[SUP] 5 6 [/SUP], Yuhei Morioka[SUP] 1 4 [/SUP], Saori Suzuki[SUP] 1 2 [/SUP], Kotaro Shirakawa[SUP] 7 [/SUP], Kei Sato[SUP] 8 9 10 11 12 13 14 [/SUP], Kumiko Yoshimatsu[SUP] 15 [/SUP], Yoshiharu Matsuura[SUP] 4 16 [/SUP], Satoshi Iwano[SUP] 17 [/SUP], Shinya Tanaka[SUP] 5 6 [/SUP], Takasuke Fukuhara[SUP] 1 2 3 4 18 [/SUP]
Affiliations
- PMID: 38638572
- PMCID: PMC11025001
- DOI: 10.1016/j.isci.2024.109647
Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome. However, NanoLuc/Furimazine and also the conventional firefly luciferase/D-luciferin are known to exhibit relatively low tissue permeability and thus less sensitivity for visualization of deep tissue including lungs. Here, we demonstrated in vivo sufficient visualization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using the pair of a codon-optimized Akaluc and AkaLumine. We engineered the codon-optimized Akaluc gene possessing the similar GC ratio of SARS-CoV-2. Using the SARS-CoV-2 recombinants carrying the codon-optimized Akaluc, we visualized in vivo infection of respiratory organs, including the tissue-specific differences associated with particular variants. Additionally, we could evaluate the efficacy of antivirals by monitoring changes in Akaluc signals. Overall, we offer an effective technology for monitoring viral dynamics in live animals.
Keywords: Methodology in biological sciences; Virology.