tetano
Editor, Senior Moderator
PLoS One. 2014 Apr 22;9(4):e95685. doi: 10.1371/journal.pone.0095685. eCollection 2014.
Investigation of avian influenza infections in wild birds, poultry and humans in eastern dongting lake, china.
Shi J1, Gao L2, Zhu Y1, Chen T1, Liu Y2, Dong L1, Liu F2, Yang H2, Cai Y3, Yu M3, Yao Y4, Xu C1, Xiao X5, Shu Y1.
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Abstract
We investigated avian influenza infections in wild birds, poultry, and humans at Eastern Dongting Lake, China. We analyzed 6,621 environmental samples, including fresh fecal and water samples, from wild birds and domestic ducks that were collected from the Eastern Dongting Lake area from November 2011 to April 2012. We also conducted two cross-sectional serological studies in November 2011 and April 2012, with 1,050 serum samples collected from people exposed to wild birds and/or domestic ducks. Environmental samples were tested for the presence of avian influenza virus (AIV) using quantitative PCR assays and virus isolation techniques. Hemagglutination inhibition assays were used to detect antibodies against AIV H5N1, and microneutralization assays were used to confirm these results. Among the environmental samples from wild birds and domestic ducks, AIV prevalence was 5.19 and 5.32%, respectively. We isolated 39 and 5 AIVs from the fecal samples of wild birds and domestic ducks, respectively. Our analysis indicated 12 subtypes of AIV were present, suggesting that wild birds in the Eastern Dongting Lake area carried a diverse array of AIVs with low pathogenicity. We were unable to detect any antibodies against AIV H5N1 in humans, suggesting that human infection with H5N1 was rare in this region.
PMID:
24755911
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24755911
Investigation of avian influenza infections in wild birds, poultry and humans in eastern dongting lake, china.
Shi J1, Gao L2, Zhu Y1, Chen T1, Liu Y2, Dong L1, Liu F2, Yang H2, Cai Y3, Yu M3, Yao Y4, Xu C1, Xiao X5, Shu Y1.
Author information
Abstract
We investigated avian influenza infections in wild birds, poultry, and humans at Eastern Dongting Lake, China. We analyzed 6,621 environmental samples, including fresh fecal and water samples, from wild birds and domestic ducks that were collected from the Eastern Dongting Lake area from November 2011 to April 2012. We also conducted two cross-sectional serological studies in November 2011 and April 2012, with 1,050 serum samples collected from people exposed to wild birds and/or domestic ducks. Environmental samples were tested for the presence of avian influenza virus (AIV) using quantitative PCR assays and virus isolation techniques. Hemagglutination inhibition assays were used to detect antibodies against AIV H5N1, and microneutralization assays were used to confirm these results. Among the environmental samples from wild birds and domestic ducks, AIV prevalence was 5.19 and 5.32%, respectively. We isolated 39 and 5 AIVs from the fecal samples of wild birds and domestic ducks, respectively. Our analysis indicated 12 subtypes of AIV were present, suggesting that wild birds in the Eastern Dongting Lake area carried a diverse array of AIVs with low pathogenicity. We were unable to detect any antibodies against AIV H5N1 in humans, suggesting that human infection with H5N1 was rare in this region.
PMID:
24755911
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24755911