tetano
Editor, Senior Moderator
Infect Genet Evol. 2015 Apr 16. pii: S1567-1348(15)00134-3. doi: 10.1016/j.meegid.2015.04.009. [Epub ahead of print]
[h=1]Intra-host viral variability in children clinically infected with H1N1 (2009) pandemic influenza.[/h] Bourret V[SUP]1[/SUP], Croville G[SUP]2[/SUP], Mansuy JM[SUP]3[/SUP], Mengelle C[SUP]3[/SUP], Mariette J[SUP]4[/SUP], Klopp C[SUP]4[/SUP], Genthon C[SUP]5[/SUP], Izopet J[SUP]3[/SUP], Gu?rin JL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Recent in-depth genetic analyses of influenza A virus samples have revealed patterns of intra-host viral genetic variability in a variety of relevant systems. These have included laboratory infected poultry, horses, pigs, chicken eggs and swine respiratory cells, as well as naturally infected poultry and horses. In humans, next generation sequencing techniques have enabled the study of genetic variability at specific positions of the viral genome. The present study investigated how 454 pyrosequencing could help unravel intra-host genetic diversity patterns on the full-length viral h?magglutinin and neuraminidase genes from human H1N1 (2009) pandemic influenza clinical cases. This approach revealed unexpected patterns of co-infection in a 3-week old toddler, arising from rapid and complex reassortment phenomena on a local epidemiological scale. It also suggested the possible existence of very low frequency mutants resistant to neuraminidase inhibitors in two untreated patients. As well as revealing patterns of intra-host viral variability, this report highlights technical challenges in the appraisal of scientifically and medically relevant topics such as the natural occurrence of homologous recombination or very low frequency drug-resistant variants in influenza virus populations.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] 2009 H1N1 pandemic; Deep sequencing; Influenza A; Intra-host viral diversity; Next generation sequencing; Pyrosequencing
PMID: 25891282 [PubMed - as supplied by publisher]
[h=1]Intra-host viral variability in children clinically infected with H1N1 (2009) pandemic influenza.[/h] Bourret V[SUP]1[/SUP], Croville G[SUP]2[/SUP], Mansuy JM[SUP]3[/SUP], Mengelle C[SUP]3[/SUP], Mariette J[SUP]4[/SUP], Klopp C[SUP]4[/SUP], Genthon C[SUP]5[/SUP], Izopet J[SUP]3[/SUP], Gu?rin JL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Recent in-depth genetic analyses of influenza A virus samples have revealed patterns of intra-host viral genetic variability in a variety of relevant systems. These have included laboratory infected poultry, horses, pigs, chicken eggs and swine respiratory cells, as well as naturally infected poultry and horses. In humans, next generation sequencing techniques have enabled the study of genetic variability at specific positions of the viral genome. The present study investigated how 454 pyrosequencing could help unravel intra-host genetic diversity patterns on the full-length viral h?magglutinin and neuraminidase genes from human H1N1 (2009) pandemic influenza clinical cases. This approach revealed unexpected patterns of co-infection in a 3-week old toddler, arising from rapid and complex reassortment phenomena on a local epidemiological scale. It also suggested the possible existence of very low frequency mutants resistant to neuraminidase inhibitors in two untreated patients. As well as revealing patterns of intra-host viral variability, this report highlights technical challenges in the appraisal of scientifically and medically relevant topics such as the natural occurrence of homologous recombination or very low frequency drug-resistant variants in influenza virus populations.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] 2009 H1N1 pandemic; Deep sequencing; Influenza A; Intra-host viral diversity; Next generation sequencing; Pyrosequencing
PMID: 25891282 [PubMed - as supplied by publisher]