Sally Furniss
Well-known member
Interaction of Polymerase Subunit PB2 and NP with Importin α1 Is a Determinant of Host Range of Influenza A Virus
G?lsah Gabriel, Astrid Herwig, Hans-Dieter Klenk<sup>*</sup>
1 Institute of Virology, Philipps University Marburg, Germany
Abstract
We have previously reported that mutations in the polymerase proteins PB1, PB2, PA, and the nucleocapsid protein NP resulting in enhanced transcription and replication activities in mammalian cells are responsible for the conversion of the avian influenza virus SC35 (H7N7) into the mouse-adapted variant SC35M. We show now that adaptive mutations D701N in PB2 and N319K in NP enhance binding of these proteins to importin α1 in mammalian cells. Enhanced binding was paralleled by transient nuclear accumulation and cytoplasmic depletion of importin α1 as well as increased transport of PB2 and NP into the nucleus of mammalian cells. In avian cells, enhancement of importin α1 binding and increased nuclear transport were not observed. These findings demonstrate that adaptation of the viral polymerase to the nuclear import machinery plays an important role in interspecies transmission of influenza virus.
Author Summary
The natural hosts of influenza A viruses are aquatic birds. On rare occasions these viruses may be transmitted to humans and then give rise to an influenza pandemic. Human influenza is therefore a typical re-emerging infection. Evidence is increasing that the viral polymerase, an enzyme that has to enter into the nucleus of the infected cell in order to promote replication and transcription of the viral genome, is a major determinant of host range. Thus, in a comparative study of an avian influenza strain and its mouse adapted variant we have previously shown that adaptation to mice depended exclusively on mutations in the polymerase proteins. These findings supported the concept that adaptation of the polymerase to host factors is an important mechanism underlying interspecies transmission. In the present study, we have identified importin α1, a component of the nuclear pore complex, as such a host factor. We show that adaptive mutations in polymerase subunits improve binding to importin α1 in mammalian, but not in avian cells. As a result, nuclear transport of these proteins and efficiency of replication are enhanced in mammalian cells. These observations demonstrate that the interaction of the viral polymerase with the nuclear import machinery is an important determinant of host range.
http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0040011
G?lsah Gabriel, Astrid Herwig, Hans-Dieter Klenk<sup>*</sup>
1 Institute of Virology, Philipps University Marburg, Germany
Abstract
We have previously reported that mutations in the polymerase proteins PB1, PB2, PA, and the nucleocapsid protein NP resulting in enhanced transcription and replication activities in mammalian cells are responsible for the conversion of the avian influenza virus SC35 (H7N7) into the mouse-adapted variant SC35M. We show now that adaptive mutations D701N in PB2 and N319K in NP enhance binding of these proteins to importin α1 in mammalian cells. Enhanced binding was paralleled by transient nuclear accumulation and cytoplasmic depletion of importin α1 as well as increased transport of PB2 and NP into the nucleus of mammalian cells. In avian cells, enhancement of importin α1 binding and increased nuclear transport were not observed. These findings demonstrate that adaptation of the viral polymerase to the nuclear import machinery plays an important role in interspecies transmission of influenza virus.
Author Summary
The natural hosts of influenza A viruses are aquatic birds. On rare occasions these viruses may be transmitted to humans and then give rise to an influenza pandemic. Human influenza is therefore a typical re-emerging infection. Evidence is increasing that the viral polymerase, an enzyme that has to enter into the nucleus of the infected cell in order to promote replication and transcription of the viral genome, is a major determinant of host range. Thus, in a comparative study of an avian influenza strain and its mouse adapted variant we have previously shown that adaptation to mice depended exclusively on mutations in the polymerase proteins. These findings supported the concept that adaptation of the polymerase to host factors is an important mechanism underlying interspecies transmission. In the present study, we have identified importin α1, a component of the nuclear pore complex, as such a host factor. We show that adaptive mutations in polymerase subunits improve binding to importin α1 in mammalian, but not in avian cells. As a result, nuclear transport of these proteins and efficiency of replication are enhanced in mammalian cells. These observations demonstrate that the interaction of the viral polymerase with the nuclear import machinery is an important determinant of host range.
http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0040011
