tetano
Editor, Senior Moderator
PLoS One. 2016 Apr 28;11(4):e0154461. doi: 10.1371/journal.pone.0154461. eCollection 2016.
[h=1]Influenza-Specific Antibody-Dependent Phagocytosis.[/h] Ana-Sosa-Batiz F[SUP]1[/SUP], Vanderven H[SUP]1[/SUP], Jegaskanda S[SUP]1[/SUP], Johnston A[SUP]2,[/SUP][SUP]3[/SUP], Rockman S[SUP]1,[/SUP][SUP]4[/SUP], Laurie K[SUP]5[/SUP], Barr I[SUP]5[/SUP], Reading P[SUP]1,[/SUP][SUP]5[/SUP], Lichtfuss M[SUP]1[/SUP], Kent SJ[SUP]1,[/SUP][SUP]6,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.
[h=4]METHODS:[/h] We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.
[h=4]RESULTS:[/h] We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity in vitro.
[h=4]CONCLUSION:[/h] We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection in vitro. Repeated exposure of humans to multiple IAV infections likely leads to the development of ADP that is cross-reactive to strains not previously encountered. Further analyses of the protective capacity of broadly reactive influenza-specific ADP is warranted.
PMID: 27124730 [PubMed - in process] Free full text
[h=1]Influenza-Specific Antibody-Dependent Phagocytosis.[/h] Ana-Sosa-Batiz F[SUP]1[/SUP], Vanderven H[SUP]1[/SUP], Jegaskanda S[SUP]1[/SUP], Johnston A[SUP]2,[/SUP][SUP]3[/SUP], Rockman S[SUP]1,[/SUP][SUP]4[/SUP], Laurie K[SUP]5[/SUP], Barr I[SUP]5[/SUP], Reading P[SUP]1,[/SUP][SUP]5[/SUP], Lichtfuss M[SUP]1[/SUP], Kent SJ[SUP]1,[/SUP][SUP]6,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.
[h=4]METHODS:[/h] We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.
[h=4]RESULTS:[/h] We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity in vitro.
[h=4]CONCLUSION:[/h] We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection in vitro. Repeated exposure of humans to multiple IAV infections likely leads to the development of ADP that is cross-reactive to strains not previously encountered. Further analyses of the protective capacity of broadly reactive influenza-specific ADP is warranted.
PMID: 27124730 [PubMed - in process] Free full text