tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2013 Jun 19. [Epub ahead of print]
Influenza but not H1N1 influenza immunization is associated with changes in peripheral blood NK cell subset levels.
Ju?rez-Reyes A, Noyola D, Monsiv?is-Urenda A, Alvarez-Quiroga C, Gonz?lez-Amaro R.
Source
from Departments of Immunology.
Abstract
The innate immune system constitutes the first line of defense against viral agents, and NK cells seem to have an important protective role during the early phases of influenza virus infections. We decided to assess the levels of NK and NKT lymphocytes, and the expression of different membrane receptors (NKp44, NKp46, NKG2A, KIR3DL1/DS1, KIR2DL1/DS1, and CD161) in the peripheral blood of patients with influenza (n=17) and healthy individuals immunized against this virus (seasonal and (H1N1)pdm2009 influenza vaccines, n=15 and n=12, respectively). A blood sample was obtained from all individuals and NK and NKT cells subsets were analyzed by multi-parametric flow cytometry. We found that those patients with severe influenza (n=9) showed a significant increase in the percent of NKp46+NKp44+ NK cells, and the proportion of NK and NKT lymphocytes expressing KIR2DL1 and KIR3DL1 as well as a reduction in NKp46+NKp44- NK cells, compared to the healthy controls (n=27). By contrast, influenza immunization, either against the seasonal or the pandemic H1N1 virus, was not associated with important changes in the levels of NK and NKT lymphocytes or the expression of the different receptors by these cells. Our data suggest that severe influenza is associated with important and complex alterations on NK cells, which might contribute to the pathogenesis of this condition.
PMID:
23784853
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23784853
Influenza but not H1N1 influenza immunization is associated with changes in peripheral blood NK cell subset levels.
Ju?rez-Reyes A, Noyola D, Monsiv?is-Urenda A, Alvarez-Quiroga C, Gonz?lez-Amaro R.
Source
from Departments of Immunology.
Abstract
The innate immune system constitutes the first line of defense against viral agents, and NK cells seem to have an important protective role during the early phases of influenza virus infections. We decided to assess the levels of NK and NKT lymphocytes, and the expression of different membrane receptors (NKp44, NKp46, NKG2A, KIR3DL1/DS1, KIR2DL1/DS1, and CD161) in the peripheral blood of patients with influenza (n=17) and healthy individuals immunized against this virus (seasonal and (H1N1)pdm2009 influenza vaccines, n=15 and n=12, respectively). A blood sample was obtained from all individuals and NK and NKT cells subsets were analyzed by multi-parametric flow cytometry. We found that those patients with severe influenza (n=9) showed a significant increase in the percent of NKp46+NKp44+ NK cells, and the proportion of NK and NKT lymphocytes expressing KIR2DL1 and KIR3DL1 as well as a reduction in NKp46+NKp44- NK cells, compared to the healthy controls (n=27). By contrast, influenza immunization, either against the seasonal or the pandemic H1N1 virus, was not associated with important changes in the levels of NK and NKT lymphocytes or the expression of the different receptors by these cells. Our data suggest that severe influenza is associated with important and complex alterations on NK cells, which might contribute to the pathogenesis of this condition.
PMID:
23784853
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23784853