tetano
Editor, Senior Moderator
Virology. 2017 Jun 21;509:167-177. doi: 10.1016/j.virol.2017.06.020. [Epub ahead of print]
[h=1]Influenza A virus cap-snatches host RNAs based on their abundance early after infection.[/h] Sikora D[SUP]1[/SUP], Rocheleau L[SUP]1[/SUP], Brown EG[SUP]1[/SUP], Pelchat M[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus RNA polymerase cleaves the 5' ends of host RNAs and uses these RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' host-derived ends of the eight viral mRNAs of influenza A/Puerto Rico/8/1934 (H1N1) virus in infected A549 cells, and compared the population to those of A/Hong Kong/1/1968 (H3N2) and A/WSN/1933 (H1N1). In the three strains, the viral RNAs target different populations of host RNAs. Host RNAs are cap-snatched based on their abundance, and we found that RNAs encoding proteins involved in metabolism are overrepresented in the cap-snatched populations. Because this overrepresentation could be a reflection of the host response early after infection, and thus of the increased availability of these transcripts, our results suggest that host RNAs are cap-snatched mainly based on their abundance without preferential targeting.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cap-snatching; Capped host primer profiling; High-throughput sequencing; Influenza A virus; Viral transcription
PMID: 28646652 DOI: 10.1016/j.virol.2017.06.020
[h=1]Influenza A virus cap-snatches host RNAs based on their abundance early after infection.[/h] Sikora D[SUP]1[/SUP], Rocheleau L[SUP]1[/SUP], Brown EG[SUP]1[/SUP], Pelchat M[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus RNA polymerase cleaves the 5' ends of host RNAs and uses these RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' host-derived ends of the eight viral mRNAs of influenza A/Puerto Rico/8/1934 (H1N1) virus in infected A549 cells, and compared the population to those of A/Hong Kong/1/1968 (H3N2) and A/WSN/1933 (H1N1). In the three strains, the viral RNAs target different populations of host RNAs. Host RNAs are cap-snatched based on their abundance, and we found that RNAs encoding proteins involved in metabolism are overrepresented in the cap-snatched populations. Because this overrepresentation could be a reflection of the host response early after infection, and thus of the increased availability of these transcripts, our results suggest that host RNAs are cap-snatched mainly based on their abundance without preferential targeting.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cap-snatching; Capped host primer profiling; High-throughput sequencing; Influenza A virus; Viral transcription
PMID: 28646652 DOI: 10.1016/j.virol.2017.06.020