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Infect Drug Resist . Adult Influenza Patients Diagnosed by Multiplex Polymerase Chain Reaction Tests, but Not Rapid Antigen Tests, and Managed Succe

tetano

tetano

Editor, Senior Moderator
Infect Drug Resist


. 2025 Dec 20:18:6763-6767.
doi: 10.2147/IDR.S564363. eCollection 2025. Adult Influenza Patients Diagnosed by Multiplex Polymerase Chain Reaction Tests, but Not Rapid Antigen Tests, and Managed Successfully During the 2024 to 2025 Major Influenza-Endemic Season in a Tertiary Hospital in Japan

Masafumi Seki[SUP] 1 [/SUP]



Affiliations
Abstract

We present the two adult cases of influenza diagnosed by multiplex polymerase chain reaction (PCR) in the 2024-2025 season, which was a major season of influenza in Japan. Although rapid antigen tests (RATs) were negative in these two cases, these patients were effectively treated by anti-influenza agents after influenza was definitively diagnosed by multiplex PCR. Relative low sensitivity and specificity might be shown in influenza diagnosis at bedside by RATs, therefore, these data suggest that multiplex PCR could be useful in influenza diagnosis and management during the influenza-endemic season.

Keywords: SARS-CoV-2; diagnostic accuracy; influenza; multiplex polymerase chain reaction; rapid antigen test; respiratory infections; respiratory syncytial virus.

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