tetano
Editor, Senior Moderator
Infect Dis (Lond)
. 2024 Jul 8:1-9.
doi: 10.1080/23744235.2024.2374307. Online ahead of print. Detection of active SARS-CoV-2 in cough aerosols from COVID-19 patients
Nuno Rufino de Sousa[SUP] 1 [/SUP], Lucille Margerie[SUP] 1 [/SUP], Laura Steponaviciute[SUP] 1 [/SUP], Julie Roux[SUP] 1 [/SUP], Matthew W Kinahan[SUP] 2 [/SUP], David Olsson[SUP] 3 4 [/SUP], Hilmir Ásgeirsson[SUP] 3 4 [/SUP], Klas I Udekwu[SUP] 2 5 [/SUP], Antonio Gigliotti Rothfuchs[SUP] 1 [/SUP]
Affiliations
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an airborne pathogen, but detection of infectious SARS-CoV-2 in air and in particular the introduction of the virus into the environment by different human expiratory manoeuvres is not well studied.
Objectives: The aim of this study was to investigate the presence of SARS-CoV-2 in cough from coronavirus disease of 2019 (COVID-19) in-patients and to study contamination of the virus in the patient's environment.
Methods: Detection of SARS-CoV-2 in cough was analyzed by PCR, culture and imaging. Detection in cough was compared to presence of the virus in air and on surfaces from patient rooms.
Results: Twenty-five patients in 21 rooms were included in the study. SARS-CoV-2 RNA was found in cough aerosols from 16 out of 22 patients that produced voluntary cough. As demonstrated by plaque-forming unit assays, active virus was isolated from 11 of these 16 patients. Using mainly molecular detection, the virus was also found in air, on high-contact surfaces, and no-touch surfaces from the room of the COVID-19 patients.
Conclusions: These results show that infectious SARS-CoV-2 circulating in air can originate from patient cough and should be considered against the risk of acquiring COVID-19 through inhalation.
Keywords: SARS-CoV-2; air sampling; cough; healthcare; infectious aerosols.
. 2024 Jul 8:1-9.
doi: 10.1080/23744235.2024.2374307. Online ahead of print. Detection of active SARS-CoV-2 in cough aerosols from COVID-19 patients
Nuno Rufino de Sousa[SUP] 1 [/SUP], Lucille Margerie[SUP] 1 [/SUP], Laura Steponaviciute[SUP] 1 [/SUP], Julie Roux[SUP] 1 [/SUP], Matthew W Kinahan[SUP] 2 [/SUP], David Olsson[SUP] 3 4 [/SUP], Hilmir Ásgeirsson[SUP] 3 4 [/SUP], Klas I Udekwu[SUP] 2 5 [/SUP], Antonio Gigliotti Rothfuchs[SUP] 1 [/SUP]
Affiliations
- PMID: 38975876
- DOI: 10.1080/23744235.2024.2374307
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an airborne pathogen, but detection of infectious SARS-CoV-2 in air and in particular the introduction of the virus into the environment by different human expiratory manoeuvres is not well studied.
Objectives: The aim of this study was to investigate the presence of SARS-CoV-2 in cough from coronavirus disease of 2019 (COVID-19) in-patients and to study contamination of the virus in the patient's environment.
Methods: Detection of SARS-CoV-2 in cough was analyzed by PCR, culture and imaging. Detection in cough was compared to presence of the virus in air and on surfaces from patient rooms.
Results: Twenty-five patients in 21 rooms were included in the study. SARS-CoV-2 RNA was found in cough aerosols from 16 out of 22 patients that produced voluntary cough. As demonstrated by plaque-forming unit assays, active virus was isolated from 11 of these 16 patients. Using mainly molecular detection, the virus was also found in air, on high-contact surfaces, and no-touch surfaces from the room of the COVID-19 patients.
Conclusions: These results show that infectious SARS-CoV-2 circulating in air can originate from patient cough and should be considered against the risk of acquiring COVID-19 through inhalation.
Keywords: SARS-CoV-2; air sampling; cough; healthcare; infectious aerosols.