tetano
Editor, Senior Moderator
Clin Exp Immunol. 2010 Jul 14. [Epub ahead of print]
Indication for a role of regulatory T cells for the advent of influenza A (H1N1)-related pneumonia.
Raftogiannis M, Antonopoulou A, Baziaka F, Spyridaki A, Koutoukas P, Tsaganos T, Savva A, Pistiki A, Georgitsi M, Giamarellos-Bourboulis EJ.
4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece.
Abstract
Summary Regulatory T cells (T(regs)) have an anti-inflammatory role. A former study in a limited number of patients found that absolute counts of T(regs) increase when infection by the new influenza H1N1 virus is complicated with pneumonia. These results generate the question if H1N1-related pneumonia is associated with a state of hypo-inflammation. A total of 135 patients were enrolled with blood sampling within less than 24 h from diagnosis; 23 with flu-like syndrome; 69 with uncomplicated H1N1-infection; seven with bacterial pneumonia; and 36 with H1N1-related pneumonia. T(regs) and CD14/HLA-DR co-expression were estimated by flow cytometry; concentrations of tumour necrosis factor-alpha (TNF-alpha), of interleukin (IL)-6 and of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) by an enzyme immunoassay; those of procalcitonin (PCT) by immuno-time-resolved amplified cryptate technology assay. Expression of human leucocyte antigen D-related (HLA-DR) on monocytes was similar between groups; absolute T(reg) counts were greater among patients with H1N1-related pneumonia than flu-like syndrome or H1N1-uncomplicated infection. Serum TNF-alpha of patients with bacterial pneumonia was greater than those of other groups, but IL-10 was similar between groups. Serum PCT was greater among patients with H1N1-related pneumonia and sTREM-1 among those with H1N1-related pneumonia. Regression analysis revealed that the most important factors related with the advent of pneumonia were the existence of underlying illnesses (P = 0.006) and of T(regs) equal to or above 16 mm(3) (P = 0.013). It is concluded that the advent of H1N1-related pneumonia is related to an early increase of the absolute T(reg) counts. This increase is probably not part of a hypo-inflammatory state of the host.
PMID: 20646008 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20646008
Indication for a role of regulatory T cells for the advent of influenza A (H1N1)-related pneumonia.
Raftogiannis M, Antonopoulou A, Baziaka F, Spyridaki A, Koutoukas P, Tsaganos T, Savva A, Pistiki A, Georgitsi M, Giamarellos-Bourboulis EJ.
4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece.
Abstract
Summary Regulatory T cells (T(regs)) have an anti-inflammatory role. A former study in a limited number of patients found that absolute counts of T(regs) increase when infection by the new influenza H1N1 virus is complicated with pneumonia. These results generate the question if H1N1-related pneumonia is associated with a state of hypo-inflammation. A total of 135 patients were enrolled with blood sampling within less than 24 h from diagnosis; 23 with flu-like syndrome; 69 with uncomplicated H1N1-infection; seven with bacterial pneumonia; and 36 with H1N1-related pneumonia. T(regs) and CD14/HLA-DR co-expression were estimated by flow cytometry; concentrations of tumour necrosis factor-alpha (TNF-alpha), of interleukin (IL)-6 and of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) by an enzyme immunoassay; those of procalcitonin (PCT) by immuno-time-resolved amplified cryptate technology assay. Expression of human leucocyte antigen D-related (HLA-DR) on monocytes was similar between groups; absolute T(reg) counts were greater among patients with H1N1-related pneumonia than flu-like syndrome or H1N1-uncomplicated infection. Serum TNF-alpha of patients with bacterial pneumonia was greater than those of other groups, but IL-10 was similar between groups. Serum PCT was greater among patients with H1N1-related pneumonia and sTREM-1 among those with H1N1-related pneumonia. Regression analysis revealed that the most important factors related with the advent of pneumonia were the existence of underlying illnesses (P = 0.006) and of T(regs) equal to or above 16 mm(3) (P = 0.013). It is concluded that the advent of H1N1-related pneumonia is related to an early increase of the absolute T(reg) counts. This increase is probably not part of a hypo-inflammatory state of the host.
PMID: 20646008 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20646008