tetano
Editor, Senior Moderator
J Virol. 2013 May 22. [Epub ahead of print]
In vivo bioluminescent imaging of influenza A virus infection and characterization of novel cross-protective monoclonal antibodies.
Heaton NS, Leyva-Grado VH, Tan GS, Eggink D, Hai R, Palese P.
Source
Icahn School of Medicine at Mount Sinai, Department of Microbiology, 1 Gustave L. Levy Place, Department of Microbiology, Annenberg Bldg-16-20, New York, NY 10029.
Abstract
Influenza A virus is a major human pathogen responsible for seasonal epidemics as well as pandemic outbreaks. Due to the continuing burden on human health, the need for new tools to study influenza pathogenesis as well as to evaluate new therapeutics is paramount. We report the development of a stable, replication competent luciferase reporter influenza A virus that can be used for in vivo imaging of viral replication. This imaging is non-invasive and allows for the longitudinal monitoring of infection in living animals. We used this tool to characterize novel monoclonal antibodies that bind the conserved stalk domain of the viral hemagglutinin of H1 and H5 subtypes and protect mice from lethal disease. The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future anti-viral therapies.
PMID:
23698304
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23698304
In vivo bioluminescent imaging of influenza A virus infection and characterization of novel cross-protective monoclonal antibodies.
Heaton NS, Leyva-Grado VH, Tan GS, Eggink D, Hai R, Palese P.
Source
Icahn School of Medicine at Mount Sinai, Department of Microbiology, 1 Gustave L. Levy Place, Department of Microbiology, Annenberg Bldg-16-20, New York, NY 10029.
Abstract
Influenza A virus is a major human pathogen responsible for seasonal epidemics as well as pandemic outbreaks. Due to the continuing burden on human health, the need for new tools to study influenza pathogenesis as well as to evaluate new therapeutics is paramount. We report the development of a stable, replication competent luciferase reporter influenza A virus that can be used for in vivo imaging of viral replication. This imaging is non-invasive and allows for the longitudinal monitoring of infection in living animals. We used this tool to characterize novel monoclonal antibodies that bind the conserved stalk domain of the viral hemagglutinin of H1 and H5 subtypes and protect mice from lethal disease. The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future anti-viral therapies.
PMID:
23698304
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23698304