tetano
Editor, Senior Moderator
Antiviral Res. 2011 Aug 16. [Epub ahead of print]
In vitro inhibitory effect of carrageenan oligosaccharide on influenza A H1N1 virus.
Wang W, Zhang P, Hao C, Zhang XE, Cui ZQ, Guan HS.
Source
Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, Ocean University of China, Qingdao 266003, PR China.
Abstract
Carrageenan polysaccharide has been reported to be able to inhibit the infection and replication of many different kinds of viruses. Here, we demonstrated that a 2kDa κ-carrageenan oligosaccharide (CO-1) derived from the carrageenan polysaccharide, effectively inhibited influenza A (H1N1) virus replication in MDCK cells (selectivity index >25.0). Moreover, the 2kDa CO-1 inhibited influenza A virus (IAV) replication better than that of 3kDa and 5kDa κ-carrageenan oligosaccharides (CO-2 and CO-3). IAV multiplication was suppressed by carrageenan oligosaccharide treatment in a dose-dependent manner. Carrageenan oligosaccharide CO-1 did not bind to the cell surface of MDCK cells but inactivated virus particles after pretreatment. Different to the actions of carrageenan polysaccharide, CO-1 could enter into MDCK cells and did not interfere with IAV adsorption. CO-1 also inhibited IAV mRNA and protein expression after its internalization into cells. Moreover, carrageenan oligosaccharide CO-1 had an antiviral effect on IAV replication subsequent to viral internalization but prior to virus release in one replication cycle. Therefore, inhibition of IAV intracellular replication by carrageenan oligosaccharide might be an alternative approach for anti-influenza A virus therapy.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21867732
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21867732
In vitro inhibitory effect of carrageenan oligosaccharide on influenza A H1N1 virus.
Wang W, Zhang P, Hao C, Zhang XE, Cui ZQ, Guan HS.
Source
Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, Ocean University of China, Qingdao 266003, PR China.
Abstract
Carrageenan polysaccharide has been reported to be able to inhibit the infection and replication of many different kinds of viruses. Here, we demonstrated that a 2kDa κ-carrageenan oligosaccharide (CO-1) derived from the carrageenan polysaccharide, effectively inhibited influenza A (H1N1) virus replication in MDCK cells (selectivity index >25.0). Moreover, the 2kDa CO-1 inhibited influenza A virus (IAV) replication better than that of 3kDa and 5kDa κ-carrageenan oligosaccharides (CO-2 and CO-3). IAV multiplication was suppressed by carrageenan oligosaccharide treatment in a dose-dependent manner. Carrageenan oligosaccharide CO-1 did not bind to the cell surface of MDCK cells but inactivated virus particles after pretreatment. Different to the actions of carrageenan polysaccharide, CO-1 could enter into MDCK cells and did not interfere with IAV adsorption. CO-1 also inhibited IAV mRNA and protein expression after its internalization into cells. Moreover, carrageenan oligosaccharide CO-1 had an antiviral effect on IAV replication subsequent to viral internalization but prior to virus release in one replication cycle. Therefore, inhibition of IAV intracellular replication by carrageenan oligosaccharide might be an alternative approach for anti-influenza A virus therapy.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21867732
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21867732