tetano
Editor, Senior Moderator
J Clin Microbiol. 2016 Oct 19. pii: JCM.01805-16. [Epub ahead of print]
[h=1]Improved Detection of Respiratory Pathogens Using High-Quality Sputum with TaqMan Array Card Technology.[/h] Wolff BJ[SUP]1[/SUP], Bramley AM[SUP]2[/SUP], Thurman KA[SUP]1[/SUP], Whitney CG[SUP]1[/SUP], Whitaker B[SUP]3[/SUP], Self WH[SUP]4[/SUP], Arnold SR[SUP]5,[/SUP][SUP]6[/SUP], Trabue C[SUP]5[/SUP], Wunderink RG[SUP]7[/SUP], McCullers J[SUP]5,[/SUP][SUP]6,[/SUP][SUP]8[/SUP], Edwards KM[SUP]4[/SUP], Jain S[SUP]2[/SUP], Winchell JM[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] New diagnostic platforms often use naso- or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multi-pathogen testing to high-quality sputum specimens to determine if more pathogens could be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ≤10 epithelial cells/low power field [lpf] and ≥25 white blood cells/lpf or a q-score definition of 2+) were tested by TaqMan Array Card (TAC), a multi-pathogen real-time polymerase chain reaction (PCR) detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ≥1 pathogen detected compared with NP/OP specimens in children (93% v. 68%, P<0.0001) and adults (88% v. 61%; P<0.0001); for each pathogen targeted, crossing threshold (Ct) values were earlier in sputum. Both bacterial (361 vs. 294) and viral detections (245 vs. 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27795345 DOI: 10.1128/JCM.01805-16
[PubMed - as supplied by publisher]
[h=1]Improved Detection of Respiratory Pathogens Using High-Quality Sputum with TaqMan Array Card Technology.[/h] Wolff BJ[SUP]1[/SUP], Bramley AM[SUP]2[/SUP], Thurman KA[SUP]1[/SUP], Whitney CG[SUP]1[/SUP], Whitaker B[SUP]3[/SUP], Self WH[SUP]4[/SUP], Arnold SR[SUP]5,[/SUP][SUP]6[/SUP], Trabue C[SUP]5[/SUP], Wunderink RG[SUP]7[/SUP], McCullers J[SUP]5,[/SUP][SUP]6,[/SUP][SUP]8[/SUP], Edwards KM[SUP]4[/SUP], Jain S[SUP]2[/SUP], Winchell JM[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] New diagnostic platforms often use naso- or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multi-pathogen testing to high-quality sputum specimens to determine if more pathogens could be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ≤10 epithelial cells/low power field [lpf] and ≥25 white blood cells/lpf or a q-score definition of 2+) were tested by TaqMan Array Card (TAC), a multi-pathogen real-time polymerase chain reaction (PCR) detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ≥1 pathogen detected compared with NP/OP specimens in children (93% v. 68%, P<0.0001) and adults (88% v. 61%; P<0.0001); for each pathogen targeted, crossing threshold (Ct) values were earlier in sputum. Both bacterial (361 vs. 294) and viral detections (245 vs. 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27795345 DOI: 10.1128/JCM.01805-16
[PubMed - as supplied by publisher]