tetano
Editor, Senior Moderator
Virology. 2014 Jul 30;464-465C:177-183. doi: 10.1016/j.virol.2014.07.013. [Epub ahead of print]
Impaired heterologous immunity in aged ferrets during sequential influenza A H1N1 infection.
Paquette SG1, Huang SS2, Banner D3, Xu L3, Leόn A3, Kelvin AA4, Kelvin DJ5.
Author information
Abstract
The major burden of influenza morbidity resides within the elderly population. The challenge managing influenza-associated illness in the elderly is the decline of immune function, where mechanisms leading to immunological senescence have not been elucidated. To better represent the immune environment, we investigated clinical morbidity and immune function during sequential homologous and heterologous H1N1 influenza infection in an aged ferret model. Our findings demonstrated experimentally that aged ferrets had significant morbidity during monosubtypic heterologous 2? challenge with significant weight loss and respiratory symptoms. Furthermore, increased clinical morbidity was associated with slower and shorter hemagglutinin antibody generation and attenuated type 1 T-cell gene responses in peripheral blood. These results revealed dampened immune activation during sequential influenza infection in aged ferrets. With the presence of an aged model, dissecting clinical morbidity, viral dynamics and immune response during influenza infection will aid the development of future prophylactics such as age specific influenza vaccines.
Copyright ? 2014 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Aging; Ferret model; Heterologous immunity; Humoral response; Immunological senescence; Influenza; Influenza A H1N1; Pandemic influenza; T-cells
PMID:
25086242
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25086242
Impaired heterologous immunity in aged ferrets during sequential influenza A H1N1 infection.
Paquette SG1, Huang SS2, Banner D3, Xu L3, Leόn A3, Kelvin AA4, Kelvin DJ5.
Author information
Abstract
The major burden of influenza morbidity resides within the elderly population. The challenge managing influenza-associated illness in the elderly is the decline of immune function, where mechanisms leading to immunological senescence have not been elucidated. To better represent the immune environment, we investigated clinical morbidity and immune function during sequential homologous and heterologous H1N1 influenza infection in an aged ferret model. Our findings demonstrated experimentally that aged ferrets had significant morbidity during monosubtypic heterologous 2? challenge with significant weight loss and respiratory symptoms. Furthermore, increased clinical morbidity was associated with slower and shorter hemagglutinin antibody generation and attenuated type 1 T-cell gene responses in peripheral blood. These results revealed dampened immune activation during sequential influenza infection in aged ferrets. With the presence of an aged model, dissecting clinical morbidity, viral dynamics and immune response during influenza infection will aid the development of future prophylactics such as age specific influenza vaccines.
Copyright ? 2014 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Aging; Ferret model; Heterologous immunity; Humoral response; Immunological senescence; Influenza; Influenza A H1N1; Pandemic influenza; T-cells
PMID:
25086242
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25086242