tetano
Editor, Senior Moderator
Immun Ageing
. 2023 Dec 18;20(1):76.
doi: 10.1186/s12979-023-00401-4. Blood circulating bacterial DNA in hospitalized old COVID-19 patients
Robertina Giacconi[SUP] 1 [/SUP], Patrizia D'Aquila[SUP] 2 [/SUP], Maurizio Cardelli[SUP] 1 [/SUP], Francesco Piacenza[SUP] 1 [/SUP], Elisa Pierpaoli[SUP] 1 [/SUP], Giada Sena[SUP] 2 [/SUP], Mirko Di Rosa[SUP] 3 [/SUP], Anna Rita Bonfigli[SUP] 4 [/SUP], Roberta Galeazzi[SUP] 5 [/SUP], Antonio Cherubini[SUP] 6 [/SUP], Massimiliano Fedecostante[SUP] 6 [/SUP], Riccardo Sarzani[SUP] 7 [/SUP], Chiara Di Pentima[SUP] 7 [/SUP], Piero Giordano[SUP] 7 [/SUP], Roberto Antonicelli[SUP] 8 [/SUP], Fabrizia Lattanzio[SUP] 4 [/SUP], Giuseppe Passarino[SUP] 2 [/SUP], Mauro Provinciali[SUP] #[/SUP][SUP] 1 [/SUP], Dina Bellizzi[SUP] #[/SUP][SUP] 9 [/SUP]
Affiliations
Background: Coronavirus disease COVID-19 is a heterogeneous condition caused by SARS-CoV-2 infection. Generally, it is characterized by interstitial pneumonia that can lead to impaired gas-exchange, acute respiratory failure, and death, although a complex disorder of multi-organ dysfunction has also been described. The pathogenesis is complex, and a variable combination of factors has been described in critically ill patients. COVID-19 is a particular risk for older persons, particularly those with frailty and comorbidities. Blood bacterial DNA has been reported in both physiological and pathological conditions and has been associated with some haematological and laboratory parameters but, to date, no study has characterized it in hospitalized old COVID-19 patients The present study aimed to establish an association between blood bacterial DNA (BB-DNA) and clinical severity in old COVID-19 patients.
Results: BB-DNA levels were determined, by quantitative real-time PCRs targeting the 16S rRNA gene, in 149 hospitalized older patients (age range 65-99 years) with COVID-19. Clinical data, including symptoms and signs of infection, frailty status, and comorbidities, were assessed. BB-DNA was increased in deceased patients compared to discharged ones, and Cox regression analysis confirmed an association between BB-DNA and in-hospital mortality. Furthermore, BB-DNA was positively associated with the neutrophil count and negatively associated with plasma IFN-alpha. Additionally, BB-DNA was associated with diabetes.
Conclusions: The association of BB-DNA with mortality, immune-inflammatory parameters and diabetes in hospitalized COVID-19 patients suggests its potential role as a biomarker of unfavourable outcomes of the disease, thus it could be proposed as a novel prognostic marker in the assessment of acute COVID-19 disease.
Keywords: Aging; COVID-19; Circulating bacterial DNA; Hospitalization; Inflammation; Mortality.
. 2023 Dec 18;20(1):76.
doi: 10.1186/s12979-023-00401-4. Blood circulating bacterial DNA in hospitalized old COVID-19 patients
Robertina Giacconi[SUP] 1 [/SUP], Patrizia D'Aquila[SUP] 2 [/SUP], Maurizio Cardelli[SUP] 1 [/SUP], Francesco Piacenza[SUP] 1 [/SUP], Elisa Pierpaoli[SUP] 1 [/SUP], Giada Sena[SUP] 2 [/SUP], Mirko Di Rosa[SUP] 3 [/SUP], Anna Rita Bonfigli[SUP] 4 [/SUP], Roberta Galeazzi[SUP] 5 [/SUP], Antonio Cherubini[SUP] 6 [/SUP], Massimiliano Fedecostante[SUP] 6 [/SUP], Riccardo Sarzani[SUP] 7 [/SUP], Chiara Di Pentima[SUP] 7 [/SUP], Piero Giordano[SUP] 7 [/SUP], Roberto Antonicelli[SUP] 8 [/SUP], Fabrizia Lattanzio[SUP] 4 [/SUP], Giuseppe Passarino[SUP] 2 [/SUP], Mauro Provinciali[SUP] #[/SUP][SUP] 1 [/SUP], Dina Bellizzi[SUP] #[/SUP][SUP] 9 [/SUP]
Affiliations
- PMID: 38111002
- DOI: 10.1186/s12979-023-00401-4
Background: Coronavirus disease COVID-19 is a heterogeneous condition caused by SARS-CoV-2 infection. Generally, it is characterized by interstitial pneumonia that can lead to impaired gas-exchange, acute respiratory failure, and death, although a complex disorder of multi-organ dysfunction has also been described. The pathogenesis is complex, and a variable combination of factors has been described in critically ill patients. COVID-19 is a particular risk for older persons, particularly those with frailty and comorbidities. Blood bacterial DNA has been reported in both physiological and pathological conditions and has been associated with some haematological and laboratory parameters but, to date, no study has characterized it in hospitalized old COVID-19 patients The present study aimed to establish an association between blood bacterial DNA (BB-DNA) and clinical severity in old COVID-19 patients.
Results: BB-DNA levels were determined, by quantitative real-time PCRs targeting the 16S rRNA gene, in 149 hospitalized older patients (age range 65-99 years) with COVID-19. Clinical data, including symptoms and signs of infection, frailty status, and comorbidities, were assessed. BB-DNA was increased in deceased patients compared to discharged ones, and Cox regression analysis confirmed an association between BB-DNA and in-hospital mortality. Furthermore, BB-DNA was positively associated with the neutrophil count and negatively associated with plasma IFN-alpha. Additionally, BB-DNA was associated with diabetes.
Conclusions: The association of BB-DNA with mortality, immune-inflammatory parameters and diabetes in hospitalized COVID-19 patients suggests its potential role as a biomarker of unfavourable outcomes of the disease, thus it could be proposed as a novel prognostic marker in the assessment of acute COVID-19 disease.
Keywords: Aging; COVID-19; Circulating bacterial DNA; Hospitalization; Inflammation; Mortality.