tetano
Editor, Senior Moderator
J Mol Biol. 2012 May 17. [Epub ahead of print]
Identifying Antigenicity Associated Sites in Highly Pathogenic H5N1 Influenza Virus Hemagglutinin by Using Sparse Learning.
Cai Z, Ducatez MF, Yang J, Zhang T, Long LP, Boon AC, Webby RJ, Wan XF.
Source
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, P.O. Box 6100, Mississippi State, MS 39762, USA.
Abstract
Since the isolation of A/goose/Guangdong/1/1996 (H5N1) in farmed geese in southern China, highly pathogenic H5N1 avian influenza viruses have posed a continuous threat to both public and animal health. The non-synonymous mutation of the H5 hemagglutinin gene has resulted in antigenic drift, leading to difficulties in both clinical diagnosis and vaccine strain selection. Characterizing H5N1's antigenic profiles would help resolve these problems. In this study, a novel sparse learning method was developed to identify antigenicity associated sites in influenza A viruses on the basis of immunologic datasets (i.e., from hemagglutination inhibition and microneutralization assays) and HA protein sequences. Twenty-one potential antigenicity associated sites were identified. A total of seventeen H5N1 mutants were used to validate the effects of eleven of these predicted sites on H5N1's antigenicity, including seven newly identified sites not located in reported antibody binding sites. The experimental data confirmed that mutations of these tested sites lead to changes in viral antigenicity, validating our method.
Copyright ? 2012 Elsevier Inc. All rights reserved.
PMID:
22609437
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22609437
Identifying Antigenicity Associated Sites in Highly Pathogenic H5N1 Influenza Virus Hemagglutinin by Using Sparse Learning.
Cai Z, Ducatez MF, Yang J, Zhang T, Long LP, Boon AC, Webby RJ, Wan XF.
Source
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, P.O. Box 6100, Mississippi State, MS 39762, USA.
Abstract
Since the isolation of A/goose/Guangdong/1/1996 (H5N1) in farmed geese in southern China, highly pathogenic H5N1 avian influenza viruses have posed a continuous threat to both public and animal health. The non-synonymous mutation of the H5 hemagglutinin gene has resulted in antigenic drift, leading to difficulties in both clinical diagnosis and vaccine strain selection. Characterizing H5N1's antigenic profiles would help resolve these problems. In this study, a novel sparse learning method was developed to identify antigenicity associated sites in influenza A viruses on the basis of immunologic datasets (i.e., from hemagglutination inhibition and microneutralization assays) and HA protein sequences. Twenty-one potential antigenicity associated sites were identified. A total of seventeen H5N1 mutants were used to validate the effects of eleven of these predicted sites on H5N1's antigenicity, including seven newly identified sites not located in reported antibody binding sites. The experimental data confirmed that mutations of these tested sites lead to changes in viral antigenicity, validating our method.
Copyright ? 2012 Elsevier Inc. All rights reserved.
PMID:
22609437
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22609437