tetano
Editor, Senior Moderator
Hum Immunol
. 2020 Aug 13;S0198-8859(20)30377-3.
doi: 10.1016/j.humimm.2020.08.001. Online ahead of print.
Conserved HLA binding peptides from five non-structural proteins of SARS-CoV-2-An in silico glance
Jose Marchan[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus Disease 2019 (COVID-19) is a dangerous global threat that has no clinically approved treatment yet. Bioinformatics represent an outstanding approach to reveal key immunogenic regions in viral proteins. Here, five severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural proteins (NSPs) (NSP7, NSP8, NSP9, NSP12, and NSP13) were screened to identify potential human leukocyte antigen (HLA) binding peptides. These peptides showed robust viral antigenicity, immunogenicity, and a marked interaction with HLA alleles. Interestingly, several peptides showed affinity by HLA class I (HLA-I) alleles that commonly activates to natural killer (NK) cells. Notably, HLA biding peptides are conserved among SARS-CoV-2, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle Eastern respiratory syndrome coronavirus (MERS-CoV). Interestingly, HLA-I and HLA class II (HLA-II) binding peptides induced humoral and cell-mediated responses after in silico vaccination. These results may open further in vitro and in vivo investigations to develop novel therapeutic strategies against coronaviral infections.
Keywords: COVID-19; Epitopes; In silico; MERS; Non-structural proteins; SARS.
. 2020 Aug 13;S0198-8859(20)30377-3.
doi: 10.1016/j.humimm.2020.08.001. Online ahead of print.
Conserved HLA binding peptides from five non-structural proteins of SARS-CoV-2-An in silico glance
Jose Marchan[SUP] 1 [/SUP]
Affiliations
- PMID: 32888767
- DOI: 10.1016/j.humimm.2020.08.001
Abstract
Coronavirus Disease 2019 (COVID-19) is a dangerous global threat that has no clinically approved treatment yet. Bioinformatics represent an outstanding approach to reveal key immunogenic regions in viral proteins. Here, five severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural proteins (NSPs) (NSP7, NSP8, NSP9, NSP12, and NSP13) were screened to identify potential human leukocyte antigen (HLA) binding peptides. These peptides showed robust viral antigenicity, immunogenicity, and a marked interaction with HLA alleles. Interestingly, several peptides showed affinity by HLA class I (HLA-I) alleles that commonly activates to natural killer (NK) cells. Notably, HLA biding peptides are conserved among SARS-CoV-2, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle Eastern respiratory syndrome coronavirus (MERS-CoV). Interestingly, HLA-I and HLA class II (HLA-II) binding peptides induced humoral and cell-mediated responses after in silico vaccination. These results may open further in vitro and in vivo investigations to develop novel therapeutic strategies against coronaviral infections.
Keywords: COVID-19; Epitopes; In silico; MERS; Non-structural proteins; SARS.