G186V and A196T make H3 human virus more avian

[Mingus]

In this recent study, G186V or A196T make a human H3 hemagglutinin virus more adapted to embryonated chicken eggs.

We could maybe extrapolate that the reverse mutation V186V or T196A could make an avian virus more "human"... but the H5 equivalent ?
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http://www.ncbi.nlm.nih.gov/entrez/..._uids=16814431&query_hl=4&itool=pubmed_docsum

1: Vaccine. 2006 Jun 9; [Epub ahead of print] Related Articles, Links
Click here to read

Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs.

Lu B, Zhou H, Chan W, Kemble G, Jin H.

MedImmune Vaccines, Inc., 297N. Bernardo Avenue, Mountain View, CA 94043, United States.

Most of the recently circulating H3N2 influenza A strains do not replicate well in embryonated chicken eggs and had to be isolated by cell culture, which presents a great challenge for influenza vaccine production using embryonated chicken eggs. We previously reported that a human H3N2 virus, A/Fujian/411/02, which replicates poorly in eggs, could be improved by changing a minimum of two HA residues (G186V/V226I or H183L/V226A). Here, we extended our work to the A/Singapore/21/04 strain that was also unable to grow in eggs. We showed that a single amino acid substitution of either G186V or A196T in the HA resulted in significantly increased virus replication in eggs without affecting virus antigenicity.

PMID: 16814431 [PubMed - as supplied by publisher]

 

[JJackson]

Re: G186V and A196T make H3 human virus more avian

Mingus, do you know if these fall within ranges with a known function on H3 e.g. binding or cleavage sites? Is H3 much the same as H5 in this area?

 

[AlaskaDenise]

Re: G186V and A196T make H3 human virus more avian

remember there was one strain of 1918 H1N1 (NY1) that effectively bound to humans with only one mutation at position 190.

see http://66.102.7.104/search?q=cache:aZhATOni1N0J:www.cofc.edu/~delliss/VirSeminar/Virology%2520articles/Influenza/flu%2520receptor.pdf+A/New+York/1/1918&hl=en&gl=us&ct=clnk&cd=3

....A/New York/1/18 HA.....allows it to bind to both 2,3 and 2,6 receptors........

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