tetano
Editor, Senior Moderator
Front Microbiol
. 2024 Dec 18:15:1513474.
doi: 10.3389/fmicb.2024.1513474. eCollection 2024. Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection
Yu Guo[SUP] #[/SUP][SUP] 1 2 [/SUP], Jing Cheng[SUP] #[/SUP][SUP] 2 [/SUP], Shuai Zhang[SUP] 1 [/SUP], Yuanyuan Zhang[SUP] 2 [/SUP], Yuzhu Zuo[SUP] 1 [/SUP], Tao Liu[SUP] 1 [/SUP], Yun Wang[SUP] 1 3 [/SUP], Chun Yang[SUP] 2 [/SUP], Chunjie Cheng[SUP] 2 [/SUP], Jinghui Fan[SUP] 1 [/SUP], Haijun Jiang[SUP] 2 [/SUP]
Affiliations
To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus in vivo, decreased the incidence of infection and conferred protection effects.
Keywords: H9N2 avian influenza virus; HA protein; avian metapneumonovirus/C; enhance green fluorescent protein; immune protection.
. 2024 Dec 18:15:1513474.
doi: 10.3389/fmicb.2024.1513474. eCollection 2024. Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection
Yu Guo[SUP] #[/SUP][SUP] 1 2 [/SUP], Jing Cheng[SUP] #[/SUP][SUP] 2 [/SUP], Shuai Zhang[SUP] 1 [/SUP], Yuanyuan Zhang[SUP] 2 [/SUP], Yuzhu Zuo[SUP] 1 [/SUP], Tao Liu[SUP] 1 [/SUP], Yun Wang[SUP] 1 3 [/SUP], Chun Yang[SUP] 2 [/SUP], Chunjie Cheng[SUP] 2 [/SUP], Jinghui Fan[SUP] 1 [/SUP], Haijun Jiang[SUP] 2 [/SUP]
Affiliations
- PMID: 39744386
- PMCID: PMC11688360
- DOI: 10.3389/fmicb.2024.1513474
To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus in vivo, decreased the incidence of infection and conferred protection effects.
Keywords: H9N2 avian influenza virus; HA protein; avian metapneumonovirus/C; enhance green fluorescent protein; immune protection.