tetano
Editor, Senior Moderator
Front Immunol
. 2021 Dec 14;12:732756.
doi: 10.3389/fimmu.2021.732756. eCollection 2021.
A Recent Update on Advanced Molecular Diagnostic Techniques for COVID-19 Pandemic: An Overview
Akanksha Roberts[SUP] 1 [/SUP], Raghuraj Singh Chouhan[SUP] 2 [/SUP], Deepshikha Shahdeo[SUP] 1 [/SUP], Narlawar Sagar Shrikrishna[SUP] 1 [/SUP], Veerbhan Kesarwani[SUP] 1 [/SUP], Milena Horvat[SUP] 2 [/SUP], Sonu Gandhi[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its related variants on time with precision and accuracy. Currently, the gold standard for detection of SARS-CoV-2 is Reverse Transcription Polymerase Chain Reaction (RT-PCR), but it lacks accuracy, is time-consuming and cumbersome, and fails to detect multi-variant forms of the virus. Herein, we have summarized conventional diagnostic methods such as Chest-CT (Computed Tomography), RT-PCR, Loop Mediated Isothermal Amplification (LAMP), Reverse Transcription-LAMP (RT-LAMP), as well new modern diagnostics such as CRISPR-Cas-based assays, Surface Enhanced Raman Spectroscopy (SERS), Lateral Flow Assays (LFA), Graphene-Field Effect Transistor (GraFET), electrochemical sensors, immunosensors, antisense oligonucleotides (ASOs)-based assays, and microarrays for SARS-CoV-2 detection. This review will also provide an insight into an ongoing research and the possibility of developing more economical tools to tackle the COVID-19 pandemic.
Keywords: CRISPR-Cas; LAMP; RT-PCR; SARS-CoV-2; biosensors; diagnostics; point of care.
. 2021 Dec 14;12:732756.
doi: 10.3389/fimmu.2021.732756. eCollection 2021.
A Recent Update on Advanced Molecular Diagnostic Techniques for COVID-19 Pandemic: An Overview
Akanksha Roberts[SUP] 1 [/SUP], Raghuraj Singh Chouhan[SUP] 2 [/SUP], Deepshikha Shahdeo[SUP] 1 [/SUP], Narlawar Sagar Shrikrishna[SUP] 1 [/SUP], Veerbhan Kesarwani[SUP] 1 [/SUP], Milena Horvat[SUP] 2 [/SUP], Sonu Gandhi[SUP] 1 [/SUP]
Affiliations
- PMID: 34970254
- PMCID: PMC8712736
- DOI: 10.3389/fimmu.2021.732756
Abstract
Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its related variants on time with precision and accuracy. Currently, the gold standard for detection of SARS-CoV-2 is Reverse Transcription Polymerase Chain Reaction (RT-PCR), but it lacks accuracy, is time-consuming and cumbersome, and fails to detect multi-variant forms of the virus. Herein, we have summarized conventional diagnostic methods such as Chest-CT (Computed Tomography), RT-PCR, Loop Mediated Isothermal Amplification (LAMP), Reverse Transcription-LAMP (RT-LAMP), as well new modern diagnostics such as CRISPR-Cas-based assays, Surface Enhanced Raman Spectroscopy (SERS), Lateral Flow Assays (LFA), Graphene-Field Effect Transistor (GraFET), electrochemical sensors, immunosensors, antisense oligonucleotides (ASOs)-based assays, and microarrays for SARS-CoV-2 detection. This review will also provide an insight into an ongoing research and the possibility of developing more economical tools to tackle the COVID-19 pandemic.
Keywords: CRISPR-Cas; LAMP; RT-PCR; SARS-CoV-2; biosensors; diagnostics; point of care.