tetano
Editor, Senior Moderator
Front Cell Infect Microbiol
. 2025 Sep 30:15:1669328.
doi: 10.3389/fcimb.2025.1669328. eCollection 2025. Analysis of co-infection in severe and critical patients with influenza A (H1N1) pneumonia using metagenomic next-generation sequencing on bronchoalveolar lavage samples
Weitao Gong[SUP] #[/SUP][SUP] 1 [/SUP], Xiaolin Ma[SUP] #[/SUP][SUP] 2 [/SUP], Gaoming Wang[SUP] #[/SUP][SUP] 3 [/SUP], Yongzhong Guo[SUP] 1 [/SUP], Zhiyuan Zhuo[SUP] 1 [/SUP], Conghui Han[SUP] 4 [/SUP], Yanmin Wu[SUP] 1 [/SUP]
Affiliations
Objectives: The study aimed to clarify the co-infection patterns in adult patients with severe influenza A (H1N1) pneumonia using Metagenomic Next-Generation Sequencing (mNGS) and to examine their impact on clinical outcomes, particularly focusing on the differences between severe and critical patient groups.
Methods: This retrospective analysis evaluated bronchoalveolar lavage fluid (BALF) from 53 adult patients diagnosed with severe influenza A (H1N1) pneumonia. Patients were categorized into severe and critical groups depending on the need for invasive ventilation. mNGS was utilized to detect and analyze co-infections, which included fungal, bacterial and viral pathogens. Statistical analysis was conducted to assess the prevalence of these co-infections and their association with clinical outcomes, such as 28-day mortality.
Results: In the cohort, 48 patients (90.6%) experienced co-infections. In the severe group, fungal infections were noted in 14 patients (66.7%), bacterial in 4 patients (19.0%), and viral in 11 patients (52.4%). Among the critical group, 22 patients (68.8%) had fungal, 23 patients (71.9%) had bacterial, and 10 patients (31.3%) had viral co-infections. There was a significantly higher incidence of co-infections in critical patients (P = 0.0002), with notable differences in Acinetobacter baumannii prevalence between the groups (P = 0.0339). Aspergillus emerged as the predominant fungal genus across the study. Septic shock (odds ratio [OR] 33.63[4.29-538.3]; P = 0.003) and fungal co-infection (OR 24.42[1.98-810.6]; P = 0.029) were identified as independent risk factors for 28-day mortality.
Conclusion: The study revealed a high rate of co-infections in both severe and critical patients suffering from influenza A (H1N1) pneumonia, with a higher frequency of bacterial infections in critical patients. Importantly, septic shock and fungal co-infections were independently associated with increased 28-day mortality, highlighting the need for monitoring and management of co-infections in these patients.
Keywords: bronchoalveolar lavage; bronchoalveolar lavage fluid; co-infection; influenza A (H1N1); metagenomicnext-generation sequencing.
. 2025 Sep 30:15:1669328.
doi: 10.3389/fcimb.2025.1669328. eCollection 2025. Analysis of co-infection in severe and critical patients with influenza A (H1N1) pneumonia using metagenomic next-generation sequencing on bronchoalveolar lavage samples
Weitao Gong[SUP] #[/SUP][SUP] 1 [/SUP], Xiaolin Ma[SUP] #[/SUP][SUP] 2 [/SUP], Gaoming Wang[SUP] #[/SUP][SUP] 3 [/SUP], Yongzhong Guo[SUP] 1 [/SUP], Zhiyuan Zhuo[SUP] 1 [/SUP], Conghui Han[SUP] 4 [/SUP], Yanmin Wu[SUP] 1 [/SUP]
Affiliations
- PMID: 41098901
- PMCID: PMC12518326
- DOI: 10.3389/fcimb.2025.1669328
Objectives: The study aimed to clarify the co-infection patterns in adult patients with severe influenza A (H1N1) pneumonia using Metagenomic Next-Generation Sequencing (mNGS) and to examine their impact on clinical outcomes, particularly focusing on the differences between severe and critical patient groups.
Methods: This retrospective analysis evaluated bronchoalveolar lavage fluid (BALF) from 53 adult patients diagnosed with severe influenza A (H1N1) pneumonia. Patients were categorized into severe and critical groups depending on the need for invasive ventilation. mNGS was utilized to detect and analyze co-infections, which included fungal, bacterial and viral pathogens. Statistical analysis was conducted to assess the prevalence of these co-infections and their association with clinical outcomes, such as 28-day mortality.
Results: In the cohort, 48 patients (90.6%) experienced co-infections. In the severe group, fungal infections were noted in 14 patients (66.7%), bacterial in 4 patients (19.0%), and viral in 11 patients (52.4%). Among the critical group, 22 patients (68.8%) had fungal, 23 patients (71.9%) had bacterial, and 10 patients (31.3%) had viral co-infections. There was a significantly higher incidence of co-infections in critical patients (P = 0.0002), with notable differences in Acinetobacter baumannii prevalence between the groups (P = 0.0339). Aspergillus emerged as the predominant fungal genus across the study. Septic shock (odds ratio [OR] 33.63[4.29-538.3]; P = 0.003) and fungal co-infection (OR 24.42[1.98-810.6]; P = 0.029) were identified as independent risk factors for 28-day mortality.
Conclusion: The study revealed a high rate of co-infections in both severe and critical patients suffering from influenza A (H1N1) pneumonia, with a higher frequency of bacterial infections in critical patients. Importantly, septic shock and fungal co-infections were independently associated with increased 28-day mortality, highlighting the need for monitoring and management of co-infections in these patients.
Keywords: bronchoalveolar lavage; bronchoalveolar lavage fluid; co-infection; influenza A (H1N1); metagenomicnext-generation sequencing.