tetano
Editor, Senior Moderator
Virology. 2016 Oct 13;499:369-374. doi: 10.1016/j.virol.2016.10.002. [Epub ahead of print]
[h=1]Focal adhesion kinase (FAK) regulates polymerase activity of multiple influenza A virus subtypes.[/h] Elbahesh H[SUP]1[/SUP], Bergmann S[SUP]2[/SUP], Russell CJ[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses (IAVs) cause numerous pandemics and yearly epidemics resulting in ~500,000 annual deaths globally. IAV modulates cellular signaling pathways at every step of the infection cycle. Focal adhesion kinase (FAK) has been shown to play a critical role in endosomal trafficking of influenza A viruses, yet it is unclear how FAK kinase activity regulates IAV replication. Using mini-genomes derived from H1N1, H5N1 and H7N9 viruses, we dissected RNA replication by IAVs independent of viral entry or release. Our results show FAK activity promotes efficient IAV polymerase activity and inhibiting FAK activity with a chemical inhibitor or a kinase-dead mutant significantly reduces IAV polymerase activity. Using co-immunoprecipitations and proximity ligation assays, we observed interactions between FAK and the viral nucleoprotein, supporting a direct role of FAK in IAV replication. Altogether, the data indicates that FAK kinase activity is important in promoting IAV replication by regulating its polymerase activity.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Focal adhesion kinase; Influenza A virus; Kinase-dead; Minigenome; Nucleoprotein; Polymerase activity; Proximity ligation assay; Replication
PMID: 27743963 DOI: 10.1016/j.virol.2016.10.002
[PubMed - as supplied by publisher]
[h=1]Focal adhesion kinase (FAK) regulates polymerase activity of multiple influenza A virus subtypes.[/h] Elbahesh H[SUP]1[/SUP], Bergmann S[SUP]2[/SUP], Russell CJ[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses (IAVs) cause numerous pandemics and yearly epidemics resulting in ~500,000 annual deaths globally. IAV modulates cellular signaling pathways at every step of the infection cycle. Focal adhesion kinase (FAK) has been shown to play a critical role in endosomal trafficking of influenza A viruses, yet it is unclear how FAK kinase activity regulates IAV replication. Using mini-genomes derived from H1N1, H5N1 and H7N9 viruses, we dissected RNA replication by IAVs independent of viral entry or release. Our results show FAK activity promotes efficient IAV polymerase activity and inhibiting FAK activity with a chemical inhibitor or a kinase-dead mutant significantly reduces IAV polymerase activity. Using co-immunoprecipitations and proximity ligation assays, we observed interactions between FAK and the viral nucleoprotein, supporting a direct role of FAK in IAV replication. Altogether, the data indicates that FAK kinase activity is important in promoting IAV replication by regulating its polymerase activity.
Copyright ? 2016 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Focal adhesion kinase; Influenza A virus; Kinase-dead; Minigenome; Nucleoprotein; Polymerase activity; Proximity ligation assay; Replication
PMID: 27743963 DOI: 10.1016/j.virol.2016.10.002
[PubMed - as supplied by publisher]