tetano
Editor, Senior Moderator
Virol J. 2016 Jun 4;13(1):90.
[h=1]Expression of importin-α isoforms in human nasal mucosa: implication for adaptation of avian influenza A viruses to human host.[/h] Ninpan K[SUP]1[/SUP], Suptawiwat O[SUP]1[/SUP], Boonarkart C[SUP]1[/SUP], Phuangphung P[SUP]2[/SUP], Sathirareuangchai S[SUP]2[/SUP], Uiprasertkul M[SUP]3[/SUP], Auewarakul P[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Transportation into the host cell nucleus is crucial for replication and transcription of influenza virus. The classical nuclear import is regulated by specific cellular factor, importin-α. Seven isoforms of importin-α have been identified in human. The preference of importin-α3 of avian influenza virus and -α7 isoform of human strains during replication in human cells was previously identified. In addition, both avian and human influenza viruses were shown to use importin-α1 isoform for their replication.
[h=4]FINDING:[/h] The mRNA levels of importin-α1, -α3, and -α7 isoforms in human respiratory tract was determined by real-time RT-PCR. The results indicate that mRNA level of importin-α7 was significantly higher than that of importin-α1 (p-value < 0.0001) and importin-α3 (p-value < 0.0001) isoforms in human nasal mucosa while importin-α1 was detected as the highest expression importin-α isoform in lung tissues.
[h=4]CONCLUSIONS:[/h] These results may explain the preference of importin-α7 isoforms in seasonal influenza viruses in human upper respiratory tract and may suggest a selective pressure toward importin-α7 in human respiratory tract infection of an avian virus.
[h=4]KEYWORDS:[/h] Human lung cells; Human nasal mucosa; Human respiratory tract; Importin-α1 isoform; Importin-α3 isoform; Importin-α7 isoform; Influenza A virus; Interspecies barrier
PMID: 27260303 [PubMed - as supplied by publisher] Free PMC Article
[h=1]Expression of importin-α isoforms in human nasal mucosa: implication for adaptation of avian influenza A viruses to human host.[/h] Ninpan K[SUP]1[/SUP], Suptawiwat O[SUP]1[/SUP], Boonarkart C[SUP]1[/SUP], Phuangphung P[SUP]2[/SUP], Sathirareuangchai S[SUP]2[/SUP], Uiprasertkul M[SUP]3[/SUP], Auewarakul P[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Transportation into the host cell nucleus is crucial for replication and transcription of influenza virus. The classical nuclear import is regulated by specific cellular factor, importin-α. Seven isoforms of importin-α have been identified in human. The preference of importin-α3 of avian influenza virus and -α7 isoform of human strains during replication in human cells was previously identified. In addition, both avian and human influenza viruses were shown to use importin-α1 isoform for their replication.
[h=4]FINDING:[/h] The mRNA levels of importin-α1, -α3, and -α7 isoforms in human respiratory tract was determined by real-time RT-PCR. The results indicate that mRNA level of importin-α7 was significantly higher than that of importin-α1 (p-value < 0.0001) and importin-α3 (p-value < 0.0001) isoforms in human nasal mucosa while importin-α1 was detected as the highest expression importin-α isoform in lung tissues.
[h=4]CONCLUSIONS:[/h] These results may explain the preference of importin-α7 isoforms in seasonal influenza viruses in human upper respiratory tract and may suggest a selective pressure toward importin-α7 in human respiratory tract infection of an avian virus.
[h=4]KEYWORDS:[/h] Human lung cells; Human nasal mucosa; Human respiratory tract; Importin-α1 isoform; Importin-α3 isoform; Importin-α7 isoform; Influenza A virus; Interspecies barrier
PMID: 27260303 [PubMed - as supplied by publisher] Free PMC Article