tetano
Editor, Senior Moderator
J Clin Microbiol. 2010 Sep 15. [Epub ahead of print]
Evaluation of Three Influenza A/B Real-time RT-PCR Assays and a New 2009 H1N1 Assay for Detection of Influenza viruses.
Selvaraju SB, Selvarangan R.
Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO; University of Missouri-Kansas City, School of Medicine, Kansas City, MO.
Abstract
The performance of three real-time influenza A/B RT-PCR assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1, 30 influenza A/H3, 30 2009 H1N1, 30 influenza B, and 30 influenza negative) were tested with CDC influenza A/B PCR (CDC), ProFlu(+) multiplex real-time RT-PCR assay (ProFlu(+)) and MGB Alert Influenza A/B & RSV RUO assay (MGB). A second set of 157 respiratory specimens (100, 2009 H1N1, 22 seasonal influenza A/H1, 15 seasonal influenza A/H3 and 20 influenza negative) were tested with a new lab developed 2009 H1N1 RT-PCR and CDC- 2009 H1N1 assay. The overall sensitivities for CDC, ProFlu(+), and MGB assay in detection of influenza A and B was 100%, 98.3% and 94% respectively. The ProFlu(+) assay failed to detect one Flu A/H1 and yielded one unresolved result with another FluA/H1 specimen. The MGB assay detected 84/87 (96.5%) of influenza A and B viruses and 26/30 (86.6%) of 2009 H1N1 viruses. The new laboratory developed 2009 H1N1 RT-PCR assay detected 100/100 (100%) 2009 H1N1 virus positive specimens, while the CDC's SW Inf A and SW H1 PCR assays failed to detect one and three low-positive 2009 H1N1 specimens, respectively. The CDC-influenza A/B and the newly developed 2009 H1N1 RT-PCR assay with internal control can be setup in two separate reactions in the same assay for routine clinical testing to detect influenza A and B and to specifically identify the 2009 H1N1 influenza virus.
PMID: 20844230 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20844230
Evaluation of Three Influenza A/B Real-time RT-PCR Assays and a New 2009 H1N1 Assay for Detection of Influenza viruses.
Selvaraju SB, Selvarangan R.
Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO; University of Missouri-Kansas City, School of Medicine, Kansas City, MO.
Abstract
The performance of three real-time influenza A/B RT-PCR assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1, 30 influenza A/H3, 30 2009 H1N1, 30 influenza B, and 30 influenza negative) were tested with CDC influenza A/B PCR (CDC), ProFlu(+) multiplex real-time RT-PCR assay (ProFlu(+)) and MGB Alert Influenza A/B & RSV RUO assay (MGB). A second set of 157 respiratory specimens (100, 2009 H1N1, 22 seasonal influenza A/H1, 15 seasonal influenza A/H3 and 20 influenza negative) were tested with a new lab developed 2009 H1N1 RT-PCR and CDC- 2009 H1N1 assay. The overall sensitivities for CDC, ProFlu(+), and MGB assay in detection of influenza A and B was 100%, 98.3% and 94% respectively. The ProFlu(+) assay failed to detect one Flu A/H1 and yielded one unresolved result with another FluA/H1 specimen. The MGB assay detected 84/87 (96.5%) of influenza A and B viruses and 26/30 (86.6%) of 2009 H1N1 viruses. The new laboratory developed 2009 H1N1 RT-PCR assay detected 100/100 (100%) 2009 H1N1 virus positive specimens, while the CDC's SW Inf A and SW H1 PCR assays failed to detect one and three low-positive 2009 H1N1 specimens, respectively. The CDC-influenza A/B and the newly developed 2009 H1N1 RT-PCR assay with internal control can be setup in two separate reactions in the same assay for routine clinical testing to detect influenza A and B and to specifically identify the 2009 H1N1 influenza virus.
PMID: 20844230 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20844230